ReoID

Phylogenetic sequence analysis and improved diagnostic assay systems for viruses of the family Reoviridae

 

Edited by Peter. P. C. Mertens and Houssam Attoui

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Isolates of Bluetongue virus type 26 (BTV-26)

in the dsRNA virus collection at  IAH Pirbright

 

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  Family: Reoviridae, Genus: Orbivirus, Species: Bluetongue virus serotype 26 (BTV-26)

Virus Species

 

* IAH dsRNA virus collection number

Serotype (identification method)

Place of original sample eg country town [grid reference] (date original sample was taken)

Isolated from which host species e.g cow, sheep, etc (Date of virus isolation, where, by whom)

Position of storage in reference freezers (Date added to collection)

Suppliers original Designation

Original sample material

 

Adapted to which cell types

 

Passage history of sample

 

[IAH passage history]

 * Original sample supplied to the collection by person / institution [IAH virus diagnosis, ISIS sample number]

  Comments

References

&

 [accession numbers]
KUW2010/02

(reference strain of BTV-26)		 Identified as
BTV-26 by serum neutralisation assay,

 real-time and conventional RT-PCR assay targeting Seg-2,
 
 Seg-2 sequence analyses. [ 1 ]		 Kuwait:

sample
collected 2010		 Sheep Shelf C9,
box C9α ,
rows G1-H10

Added to collection on
16th Sept 2010		 from animal No. 374 E1

[BHK2] 		Veterinary Diagnostic Laboratory and Animal Research Centre / Public Authority of Agriculture Affairs and Fish resources
(PAAF), Kuwait.
*
 Samples G1 to G4 removed from freezer A & B
*
Sample G5 & H1 removed from freezer A
 *
Note:  This strain of BTV-26 does not belong to either the major eastern or western topotypes of BTV 		 Note this strain of BTV-26 does not replicate in KC cells

[Seg-1: JN255156,

Seg-2: HM590642,

Seg-3: HM590643,

Seg-4: JN255157,

Seg-5: JN255158,

Seg-6: JN255159,

Seg-7: HM590644,

Seg-8: JN255160,

Seg-9: JN255161,

Seg-10: JN255162, 

Maan et al 2011a (1)

Maan et al 2011b(2)] 

Pullinger et al. 2015 (3)

KUW2010/19 BTV-26 Strain originally from Kuwait: (animal 374)

Sample was taken from sheep inoculated at TPI on 15Nov 2010
 with BTV-26 E1/BHK1		 Sheep Shelf C13,
box C13 beta
rows E1 to F10

added to collection
25 Mar 2011		 B3 9DPI

Isolated directly from spleen

[TPI/V2]

   Note this strain of BTV-26 does not replicate in KC cells
KUW2010/18 BTV-26 Strain originally from Kuwait: (animal 374)

Sample was taken from sheep inoculated at TPI on 15Nov 2010
 with BTV-26 E1/BHK1		 Sheep Shelf C13,
box C13 beta
rows C1 to D10

added to collection
25 Mar 2011		 B3 9DPI

Isolated directly from spleen

[TPI/V1]

   Note this strain of BTV-26 does not replicate in KC cells
KUW2010/17 BTV-26 Strain originally from Kuwait: (animal 374)

Sample was taken from sheep inoculated at TPI on 15Nov 2010
 with BTV-26 E1/BHK1		 Sheep Shelf C13,
box C13 beta
rows A1 to B10

added to collection
25 Mar 2011		 B3 9DPI

Isolated directly from blood

[TPI/BHK1/V1]

   Note this strain of BTV-26 does not replicate in KC cells
KUW2010/16 BTV-26 Strain originally from Kuwait: (animal 374)

Sample was taken from sheep inoculated at TPI on 15Nov 2010
 with BTV-26 E1/BHK1		 Sheep Shelf C13,
box C13 alpha,
rows I1 to J10

added to collection
25 Mar 2011		 B3 9DPI

Isolated directly from spleen

[TPI/BHK1/V1]

   Note this strain of BTV-26 does not replicate in KC cells
KUW2010/15 BTV-26 Strain originally from Kuwait: (animal 374)

Sample was taken from sheep inoculated at TPI on 15Nov 2010
 with BTV-26 E1/BHK1		 Sheep Shelf C13,
box C13 alpha,
rows G1 to H10

added to collection
25 Mar 2011		 B2 9DPI

Isolated directly from blood

[TPI/BHK1/V1]

   Note this strain of BTV-26 does not replicate in KC cells
KUW2010/14 BTV-26 Strain originally from Kuwait: (animal 374)

Sample was taken from sheep inoculated at TPI on 15Nov 2010
 with BTV-26 E1/BHK1		 Sheep Shelf C13,
box C13 alpha,
rows E1 to F10

added to collection
25 Mar 2011		 B1 7DPI

Isolated directly from blood

[TPI/BHK1/V1]

   Note this strain of BTV-26 does not replicate in KC cells
KUW2010/13 BTV-26 Kuwait:

sample
collected 2010		 sheep stored in l;iquid nitogen by reference lab as a reference strain for SNT   [TPI/    ]   Note this strain of BTV-26 does not replicate in KC cells
KUW2010/12 BTV-26 Strain originally from Kuwait: (animal 374)

Sample was taken from sheep inoculated at TPI on 15Nov 2010
 with BTV-26 E1/BHK1		 Sheep Shelf C13,
box C13 gamma,
rows A1 to B10

added to collection
25 Mar 2011		 B3 9DPI

Isolated directly from spleen

[TPI/BHK2]

 Vials A9, A10 and B10 were not added to freezer A & B Note this strain of BTV-26 does not replicate in KC cells
KUW2010/11 BTV-26 Strain originally from Kuwait: (animal 374)

Sample was taken from sheep inoculated at TPI on 15Nov 2010
 with BTV-26 E1/BHK1		 Sheep Shelf C13,
box C13 alpha,
rows C1 to D10

added to collection
25 Mar 2011		 B2 9DPI

Isolated directly from blood

[TPI/BHK2]

   Note this strain of BTV-26 does not replicate in KC cells
KUW2010/10 BTV-26 Strain originally from Kuwait: (animal 374)

Sample was taken from sheep inoculated at TPI on 15Nov 2010
 with BTV-26 E1/BHK1		 Sheep Shelf C13,
box C13 alpha,
rows A1 to B10

added to collection
25 Mar 2011		 B1 7DPI

Isolated directly from blood

[TPI/BHK2]

   Note this strain of BTV-26 does not replicate in KC cells
KUW2010/09 BTV-26 Strain originally from Kuwait: (animal 374)

Sample was taken from sheep inoculated at TPI on 15Nov 2010
 with BTV-26 E1/BHK1		 Sheep

Shelf C12,
box C12 gamma ,
rows C1-D10

added to collection
25 Mar 2011

 B3 9DPI

Isolated directly from blood

[TPI/BHK1]

 strain originally provided by
Veterinary Diagnostic Laboratory and Animal Research Centre / Public Authority of Agriculture Affairs and Fish resources (PAAF) Kuwait.
 *
 Vials C10 and D10  were not added to freezer A & B
*
 Vials C9 were removed from freezers A & B
*
Vial D9 was removed from freezer B
*		 Note this strain of BTV-26 does not replicate in KC cells

related to  KUW2010/06,
to
KUW2010/19
KUW2010/08 BTV-26 Strain originally from Kuwait: (animal 374)

Sample was taken from sheep inoculated at TPI on 15Nov 2010
 with BTV-26 E1/BHK1		 Sheep Shelf C12,
box C12 gamma ,
rows A1-B10

added to collection
25 Mar 2011		 B3 9DPI

Isolated directly from spleen

[TPI/BHK1]

 strain originally provided by
Veterinary Diagnostic Laboratory and Animal Research Centre / Public Authority of Agriculture Affairs and Fish resources (PAAF)Kuwait.		 Note this strain of BTV-26 does not replicate in KC cells

related to  KUW2010/06,
to
KUW2010/19
KUW2010/07 BTV-26 Strain originally from Kuwait: (animal 374)

Sample was taken from sheep inoculated at TPI on 15Nov 2010
 with BTV-26 E1/BHK1		 Sheep Shelf C12,
box C12 gamma ,
rows I1-J10

added to collection
25 Mar 2011		 B2 9DPI

Isolated directly from blood

[TPI/BHK1]

 strain originally provided by
Veterinary Diagnostic Laboratory and Animal Research Centre / Public Authority of Agriculture Affairs and Fish resources (PAAF), Kuwait.		 Note this strain of BTV-26 does not replicate in KC cells

 related to  KUW2010/06,
to
KUW2010/19
KUW2010/06 BTV-26 Strain originally from Kuwait: (animal 374)

Sample was taken from sheep inoculated at TPI on 15Nov 2010
 with BTV-26 E1/BHK1		 Sheep Shelf C12,
box C12 gamma ,
rows G1-H10

added to collection
25 Mar 2011		 B1 7DPI

Isolated directly from blood

[TPI/BHK1]

 strain originally provided by
Veterinary Diagnostic Laboratory and Animal Research Centre / Public Authority of Agriculture Affairs and Fish resources (PAAF), Kuwait.		 Note this strain of BTV-26 does not replicate in KC cells

 related to  KUW2010/06,
to
KUW2010/19
KUW2010/05 BTV-26

Identified as
BTV-26 by serum neutralisation assay, real-time and conventional RT-PCR assay targeting Seg-2 and by Seg-2 sequence analyses.
Kuwait:

sample
collected 2010		 Sheep Shelf C12,
box C12 gamma,
rows E1-F10

added to collection
25 Mar 2011		 from animal No. 374 E1

[TPI/BHK1/V1]		 Veterinary Diagnostic Laboratory and Animal Research Centre / Public Authority of Agriculture Affairs and Fish resources (PAAF), Kuwait. Note this strain of BTV-26 does not replicate in KC cells
KUW2010/04 Identified as
BTV-26 by serum neutralisation assay,

 real-time and conventional RT-PCR assay targeting Seg-2,
 
 Seg-2 sequence analyses. [ 1 ]		 Kuwait:

sample
collected 2010		 Sheep Shelf C9,
box C9α ,
rows I1-J10

Added to collection on
16th Sept 2010		 from animal No. 374 E1

[TPI/BHK3]		 Veterinary Diagnostic Laboratory and Animal Research Centre / Public Authority of Agriculture Affairs and Fish resources
(PAAF), Kuwait.
Note this strain of BTV-26 does not replicate in KC cells
KUW2010/03 Identified as
BTV-26 by serum neutralisation assay,

 real-time and conventional RT-PCR assay targeting Seg-2,
 
 Seg-2 sequence analyses. [ 1 ]		 Kuwait:

sample
collected 2010		 Sheep Shelf C9,
box C9β ,
rows A1-B10

Added to collection on
16th Sept 2010		 from animal No. 374 E1

[TPI/BHK1/VERO2]		 Veterinary Diagnostic Laboratory and Animal Research Centre / Public Authority of Agriculture Affairs and Fish resources
 (PAAF), Kuwait.

Note this strain of BTV-26 does not replicate in KC cells
KUW2010/01 Identified as
BTV-26 by serum neutralisation assay,

 real-time and conventional RT-PCR assay targeting Seg-2,
 
 Seg-2 sequence analyses. [ 1 ]		 Kuwait:

sample
collected 2010		 Sheep from animal No. 374 original blood sample Veterinary Diagnostic Laboratory and Animal Research Centre / Public Authority of Agriculture Affairs and Fish resources
(PAAF), Kuwait. 		Note this strain of BTV-26 does not replicate in KC cells

 

 

References
1.           


    		Maan S, Maan NS, Nomikou K, Batten C, Antony F, Belaganahalli MN, Samy AM, Reda AA, Al-Rashid SA, El Batel M, Oura CA, Mertens PP. (2011a) Novel bluetongue virus serotype from Kuwait. Emerg Infect Dis. 2011 May;17(5):886-9. doi: 10.3201/eid1705.101742.

2.


Maan S, Maan NS, Nomikou K, Veronesi E, Bachanek-Bankowska K, Belaganahalli MN, Attoui H, Mertens PP. (2011b) Complete genome characterisation of a novel 26th bluetongue virus serotype from Kuwait. PLoS One. 2011;6(10):e26147. doi: 0.1371/journal.pone.0026147. Epub 2011 Oct 21.

3.
Pullinger GD, Guimerą Busquets M, Nomikou K, Boyce M, Attoui H, Mertens PP. (2015) Identification of the Genome Segments of Bluetongue Virus Serotype 26 (Isolate KUW2010/02) that Restrict Replication in a Culicoides sonorensis Cell Line (KC Cells). PLoS One. 2016 Feb 18;11(2):e0149709. doi: 10.1371/journal.pone.0149709. eCollection 2016.  PMID: 26890863

 

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