ReoID

Phylogenetic sequence analysis and improved diagnostic assay systems for viruses of the family Reoviridae

 

Edited by Peter. P. C. Mertens and Houssam Attoui

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Reassortant  and modified BTV strains generated by reverse genetics

in the Orbivirus Reference Collection (ORC) at  The Pirbright Institute

 

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 Family: Reoviridae, Genus: Orbivirus, Species: Bluetongue virus (BTV)  - Rescued and genetically modified viruses generated by reverse genetics

 Unique
GM-Virus identification
 number

 BTV serotype
(based on Seg-2)

Virus genotype / parental strains

 

(virus backbone / modifications)

Position of storage in

reference freezers

 

 (Date added to collection)

Suppliers

Isolate Designation

 

Passage history

of rescued virus

Virus generated by person / institution

Date generated

 

Comments 

 

References &

 accession numbers  
BTV-RV0001 BTV-4 BTV-4 [MOR2004/02] backbone,
 with BTV-1
Mor2006/06 Seg-1 		Shelf A19,
Box A19α,
Position A1-A5		 (previously called RG1) [BSR3] Gill Pullinger / TPI
24/08/2012		  
BTV-RV0002 BTV-4 BTV-4 [MOR2004/02] backbone,
 with BTV-1
[MOR2006/06] Seg-3 		Shelf A19,
Box A19α,
Position A6-A10		 (previously called RG3) [BSR3] Gill Pullinger / TPI
24/08/2012		  
BTV-RV0003 BTV-4  BTV-4 [MOR2004/02] backbone,
 with BTV-1
[MOR2006/06] Seg-4 		Shelf A19,
 Box A19α,
Position B1-B5 		(previously called RG4) [BSR3] Gill Pullinger / TPI
24/08/2012		  
BTV-RV0004 BTV-4 BTV-4 [MOR2004/02] backbone,
with BTV-1
[MOR2006/06] Seg-5 		Shelf A19,
Box A19α,
Position B6-B10		 (previously called RG5) [BSR3] Gill Pullinger / TPI
28/08/2012		  
BTV-RV0005 BTV-4  BTV-4 [MOR2004/02] backbone,
 with BTV-1
[MOR2006/06]  Seg-6		 Shelf A19,
Box A19α,
Position C1-C5 		 (previously called RG6) [BSR3] Gill Pullinger / TPI
28/08/2012		  
BTV-RV0006 BTV-4 BTV-4 [MOR2004/02] backbone,
 with BTV-1
[MOR2006/06]  Seg-8		 Shelf A19,
 Box A19α,
Position C6-C10 		previously called RG8) [BSR3] Gill Pullinger / TPI
28/08/2012
BTV-RV0007 BTV-4 BTV-4 [MOR2004/02] backbone,
 with BTV-1
[MOR2006/06]  Seg-9		 Shelf A19,
Box A19α,
Position D1-D5 		 (previously called RG9) [BSR3] Gill Pullinger / TPI
29/08/2012
BTV-RV0008 BTV-4 BTV-4 [MOR2004/02] backbone,
 with BTV-1
[MOR2006/06]  Seg-10		 Shelf A19,
Box A19α,
Position D6-D10 		 (previously called RG10) [BSR3] Gill Pullinger / TPI
29/08/2012		  
BTV-RV0009 BTV-1 BTV-1 [MOR2006/06] backbone,
 with BTV-4
[MOR2004/02] Seg-7 		Shelf A19,
Box A19α,
Position E1-E5 		(previously called RG17) [BSR3] Gill Pullinger / TPI
30/08/2012		  
BTV-RV0010 BTV-4 Rescued BTV-4
[MOR2004/02] 		Shelf A19,
Box A19α,
Position E6-E10 		(previously called
RG BTV-4)		 [BSR3] Gill Pullinger / TPI  
BTV-RV0011 BTV-1 BTV-1 [RSArrrr/01] backbone with BTV-26
[KUW2010/ 02]  Seg-1 		Shelf A19,
Box A19α,
Position F1-F5		 (previously called RG21) [BSR3]
[Plaque cloned x4]		 Gill Pullinger / TPI
10/04/2011		 Sequencing showed that Seg-9 of RG21 [BTV-RV0011] contains an insert
BTV-RV0012 BTV-1  BTV-1 RSArrrr/01 backbone
with  BTV-26
 [KUW2010/02] Seg-3 		Shelf A19,
Box A19α,
Position F6-F10		  (previously called RG23) [BSR3] Gill Pullinger / TPI
10/04/2014		  
BTV-RV0013 BTV-1 BTV-1 [RSArrrr/01] backbone
with BTV-26
[KUW2010/02] Seg-4 		Shelf A19,
Box A19α,
Position G1-G5		 (previously called RG24) [BSR3] Gill Pullinger / TPI
28/11/2012		  
BTV-RV0014 BTV-1  BTV-1 [RSArrrr/01] backbone
with BTV-26
[KUW2010/02] Seg-5 		Shelf A19,
Box A19α,
Position G6-G10		 (previously called RG25) [BSR3] Gill Pullinger / TPI
28/11/2012		  
BTV-RV0015 BTV-1 BTV-1 [RSArrrr/01] backbone
with BTV-26
 [KUW2010/02] Seg-6		 Shelf A19,
Box A19α,
Position H1-H5 		 (previously called RG26) [BSR3] Gill Pullinger / TPI
28/11/2012
BTV-RV0016 BTV-1 BTV-1 [RSArrrr/01] backbone
with BTV-26
 [KUW2010/02] Seg-7 		Shelf A19,
Box A19α,
Position H6-H10 		(previously called RG27) [BSR3] Gill Pullinger / TPI
28/11/2012		  
BTV-RV0017 BTV-1 BTV-1 [RSArrrr/01] backbone
with BTV-26
 [KUW2010/02] Seg-8		 Shelf A19,
Box A19α,
Position I1-I5		 (previously called RG28) [BSR3] Gill Pullinger / TPI
29/11/2012		  
BTV-RV0018 BTV-1 BTV-1 [RSArrrr/01] backbone
with BTV-26
 [KUW2010/02] Seg-9 		Shelf A19,
Box A19α,
Position I6-I10		 (previously called RG29) [BSR3] Gill Pullinger / TPI
29/11/2012
BTV-RV0019 BTV-1 BTV-1 [RSArrrr/01] backbone
with BTV-26
 [KUW2010/02] Seg-10		 Shelf A19,
Box A19α,
Position J1-J5 		(previously called RG30) [BSR3] Gill Pullinger / TPI
29/11/2012		  
BTV-RV0020 BTV-26 BTV-1 [RSArrrr/01] backbone
with BTV-26
 [KUW2010/02]
Seg-2, Seg-6 and  Seg-7		 Shelf A19,
Box A19α,
Position J6-J10 		 (previously called RG2,6,7) [BSR3] Gill Pullinger / TPI
27/03/2012
  
BTV-RV0021 BTV-4 Composition: BTV-1 [MOR2006/06] backbone
with BTV-4 [MOR2004/02] Seg-2		 Shelf A19,
Box A19β,
Position A1-A5 		 (previously called RG12) [BSR3] Gill Pullinger / TPI
27/03/2012
  
BTV-RV0022 BTV-1 BTV-4 [MOR2004/02] backbone
with
BTV-1 [Mor2006/06] Seg 2, Seg-6 & Seg-7		 Shelf A19,
Box A19β,
Position A6-A10		   [BSR3] Gill Pullinger / TPI
27/03/2012
  
BTV-RV0023 BTV-1 Rescued
BTV-1 [RSArrrr/01]		 Shelf A19,
Box A19β,
Position B1-B5 		  [BSR3] Gill Pullinger / TPI
28/03/2012		  
BTV-RV0024 BTV-1 BTV-1 [RSArrrr/01]
with HA tag inserted in extracellular loop of NS3 (Seg-10-HA)		 helf A19,
Box A19β,
Position B6-B10 		  [BSR3] Gill Pullinger / TPI
28/03/2012		  
BTV-RV0025 BTV-1 BTV-1 [RSArrrr/01]
with Flag tag fused to C terminus of NS1(Seg-5-Flag)		 Shelf A19,
Box A19β,
Position C1-C5		   [BSR3] Gill Pullinger / TPI
28/03/2012		  
BTV-RV0026 BTV-1 BTV-1 [RSArrrr/01]
 with mutatedstart codon for NS3a ORF 		Shelf A19,
Box A19β,
Position C6-C10		   [BSR3] Gill Pullinger / TPI
28/03/2012		  (note: only expresses full-length NS3 not NS3a)
BTV-RV0027 BTV-1 BTV-1 [RSArrrr/01] backbone, with
BTV-26 [KUW2010/02]
 (Seg-1 and Seg-3)		 Shelf A19,
Box A19β,
Position D1-D5		   [BSR3] Gill Pullinger / TPI
10/04/2014		  
BTV-RV0028 BTV-1 BTV-1 [RSArrrr/01] backbone with
BTV-26 [KUW2010/02]
(Seg 7-VP7 P197A mutation)		 Shelf A19,
Box A19β,
Position D6-D10		   [BSR3] Marc Guimera / TPI
27/03/2012		  
BTV-RV0029 BTV-1  BTV-1 [RSArrrr/01] backbone with
BTV-26 [KUW2010/02] (Seg 7-VP7 G146A and N148A mutations)		 Shelf A19,
Box A19β,
Position E1-E5 		  [BSR3] Marc Guimera  / TPI
27/03/2012		  
BTV-RV0030 BTV-1 BTV-1 [RSArrrr/01] backbone with
BTV-26 [KUW2010/02] (Seg 7-VP7 G146A, N148A and P197A mutations)		 helf A19,
Box A19β,
Position E6-E10		 [BSR3] Marc Guimera / TPI
10/04/2014
BTV-RV0031 BTV-1 BTV-1 [RSArrrr/01] backbone
with BTV-26 [KUW2010/02]
 Seg 7-VP7ch1		 Shelf A19,
Box A19β,
 Position F1-F5 		  [BSR3] Marc Guimera  / TPI
10/04/2014		  
BTV-RV0032 BTV-1 BTV-1 [RSArrrr/01] backbone
with BTV-26 [KUW2010/02]
Seg 7-VP7ch2		 Shelf A19,
 Box A19β,
Position F6-F10		   [BSR3] Marc Guimera  / TPI
10/04/2014		  
BTV-RV0033 BTV-4 BTV-4 [MOR2004/02] backbone
with BTV-1 [MOR2006/06] Seg 7		 Shelf A19,
Box A19β,
Position G1-G5		 (previously called RG7) [BSR3] Gill Pullinger / TPI
03/05/2013		  
BTV-RV0034 BTV-1 BTV-1 [MOR2006/06] backbone
with BTV-4 [MOR2004/02] Seg 1 		Shelf A19,
Box A19β,
Position G6-G10		 (previously called RG11) [BSR3] Gill Pullinger / TPI
30/09/2013		  
BTV-RV0035 BTV-1 BTV-1 [MOR2006/06] backbone
with BTV-4 [MOR2004/02] Seg 3		 Shelf A19,
Box A19β,
Position H1-H5		  (previously called RG13) [BSR3] Gill Pullinger / TPI
30/09/2013		  
BTV-RV0036 BTV-1 BTV-1 [MOR006/06] backbone
with BTV-4 [MOR2004/02] Seg 4 		Shelf A19,
Box A19β,
 Position H6-H10		 (previously called RG14) [BSR3] Gill Pullinger / TPI
30/09/2013		  
BTV-RV0037 BTV-1 Composition: BTV-1 [MOR2006/06] backbone with BTV-4 [MOR2004/02] Seg 5 Shelf A19,
Box A19β,
Position I1-I5		 (previously called RG15) [BSR3] Gill Pullinger / TPI
27/09/2013		  
BTV-RV0038 BTV-4 BTV-1 [MOR2006/06] backbone with BTV-4 [MOR2004/02]
Seg 2, Seg-6 & Seg-7		 Shelf A19,
 Box A19β,
Position I6-I10		   [BSR3] Gill Pullinger / TPI
03/05/2014		  
BTV-RV0039 BTV-1 BTV-1 [MOR2006/06] backbone with BTV-4
 [MOR2004/02] Seg 8 		Shelf A19,
Box A19β,
Position J1-J5 		(previously called RG18) [BSR3] Gill Pullinger / TPI
03/10/2013		  
BTV-RV0040 BTV-1 BTV-1 [MOR2006/06] backbone  with BTV-4
 [MOR2004/02] Seg 9 		Shelf A19,
 Box A19β,
Position J6-J10		 (previously called RG19) [BSR3] Gill Pullinger / TPI
03/10/2013		  
BTV-RV0041 BTV-1 BTV-1 [MOR2006/06] backbone with BTV-4
 [MOR2004/02] Seg 10 		Shelf A19,
Box A19γ,
Position A1-A5		 (previously called RG20) [BSR3] Gill Pullinger / TPI
10/10/2013		  
BTV-RV0042 BTV-1  Rescued BTV-1 [MOR2006/06] Shelf A19,
Box A19γ,
Position A6-A10 		BTV-1 [BSR3] Gill Pullinger / TPI
03/10/2013		  
BTV-RV0043 BTV-1 BTV-1 [RSArrrr/01]
with  Seg-7/VP7 RGD to RGA mutant		 Shelf A19,
 Box A19γ,
Position B1-B5		   [BSR3] Gill Pullinger / TPI
12/08/2013		 Seg-7/VP7
RGD to RGA mutant
BTV-RV0044 BTV-1 BTV-1 [RSArrrr/01]
with
 BTV-26 [KUW2010/02] Seg-7 RGD to RGA mutant		 Shelf A19,
Box A19γ,
Position B6-B10 		  [BSR3] Gill Pullinger / TPI
29/07/2013		 BTV-26 Seg-7 RGD to RGA mutant
BTV-RV0046 BTV-1 BTV-1 [RSArrrr/01] backbone, with
BTV-1 A16S		 Shelf A19,
Box A19γ,
Position C6-C10 		  [BSR3] Marc Guimera / TPI
02/10/2014		 VP7 A16S
BTV-RV0047 BTV-1 BTV-1 [RSArrrr/01] backbone,  with
VP7 S117T		 Shelf A19,
Box A19γ,
Position D1-D5		   [BSR3] Marc Guimera  / TPI
02/10/2014		 VP7 S117T
BTV-RV0048 BTV-1  BTV-1[RSArrrr/01]
Seg-9/NS4 mutant		 Shelf A19,
Box A19γ,
Position D6-D10		   [BSR3] Gill Pullinger / TPI
12/12/2014		 NS4 mutant
BTV-RV0049 BTV-1 BTV-1[RSArrrr/01]
 Seg-5/NS1 Tetracysteine-tagged clone 2		 Shelf A19,
Box A19γ,
Position E1-E2 		  [BSR3] Gill Pullinger / TPI
12/12/2014		 NS1 Tetracysteine-tagged clone 2
BTV-RV0050 BTV-1  BTV-1[RSArrrr/01]
Seg-10/-NS5 mutant		 Shelf A19,
Box A19γ,
Position E6-E10 		[BSR3] Gill Pullinger / TPI
01/06/2015
 BTV-1[RSArrrr/01]
 NS5 mutant
BTV-RV0051 BTV-1  BTV-1[RSArrrr/01]Seg9/NS4,Seg-10/NS5 double mutant Shelf A19,
Box A19γ,
Position F1-F5 		  [BSR3] Gill Pullinger / TPI
01/06/2015		 BTV-1[RSArrrr/01]
 NS4, NS5
double mutant
BTV-RV0052 BTV-1 BTV-1 [RSArrrr/01]
Seg-2/VP2 Tetracysteine-tagged		 Shelf A19,
Box A19γ,
Position G6-G10 		  - [BSR2]  / TPI
10/12/2015		 BTV-1 [RSArrrr/01]
Seg-2/VP2 Tetracysteine-tagged
BTV-RV0053 BTV-1 BTV-1 [RSArrrr/01]
Seg-8/ NS2  S249A		 Shelf A19,
Box A19γ,
Position H1-H5		 - [BSR3]  / TPI
10/12/2015		 aa position 249 of NS2  changed
from S to A
BTV-RV0054 BTV-1 BTV-1 [RSArrrr/01]
 Seg-8/ NS2  S259A		 Shelf A19,
Box A19γ,
Position H6-H10		 - [BSR3]  / TPI
10/12/2015		 aa position 259 of NS2  changed
from S to A
BTV-RV0055 BTV-1 BTV-1 [RSArrrr/01]
with Seg-1of BTV-26 [KUW2010/02], and Seg-9 of RG21 (RSA-1 Seg-9 with insertion)		 Shelf A19,
Box A19γ,
Position I1-I5		 - [BSR4] Marc Guimera / TPI
18/01/2016		 Sequencing showed that Seg-9 of RG21 [BTV-RV0011] contains an insert
BTV-RV0056 BTV-1 BTV-1 [RSArrrr/01]
 with BTV-1 Seg-7/VP7 A262S, T264A		 Shelf A19,
Box A19γ,
Position I6-I10		 - [BSR3] Marc Guimera / TPI
18/01/2016		 aa 262 of VP7 changed
from A to S
&
aa 264 changed
from T to A
BTV-RV0057 BTV-1  BTV-1 [RSArrrr/01]
with BTV-1 Seg-7/VP7 N302S, D305E		 Shelf A19,
Box A19γ,
Position  J1-J5		 - [BSR3] Marc Guimera / TPI
18/01/2016		 aa 302 of VP7 changed
from N to S
&
aa 305 changed
from D to E
BTV-RV0058 BTV-1  BTV-1 [RSArrrr/01]
with BTV-1 Seg-7/VP7 L310I		 Shelf A19,
Box A19γ,
Position  J6-J10		 - [BSR3] Marc Guimera / TPI
18/01/2016		 aa 310 of VP7 changed
from L to I
BTV-RV0059 BTV-1

BTV-1 [RSArrrr/01] backbone

 with BTV-1 Seg-7/VP7 P334A

 Shelf A20,
Box A20α,
Position A1-A5		 - [BSR3] Marc Guimera / TPI
18/01/2016		 aa 334 of VP7 changed
from P to A
BTV-RV0060 BTV-1 BTV-1 RSArrrr/01 backbone
with BTV-26 VP7 V85T		 Shelf A20,
Box A20α,
Position A6-A10 		- [BSR3] Marc Guimera / TPI
02/02/2016		 5 aliquots in total in each freezer
aa 85 of VP7 changed
from V to T
               
               
               
               
               

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