|
Family: Reoviridae, Genus: Orbivirus,
Species:
Bluetongue virus
(BTV)
serotype 2 |
|
*IAH
dsRNA virus collection number
( ICTVdb
isolate Accession Number) |
Virus Species
&
Serotype
(identification method) |
Place of original sample eg country
town
[grid reference]
(date
original sample was taken) |
Isolated from which host species e.g
cow, sheep, etc
(Date of virus isolation, where, by
whom) |
Position of storage in reference
freezers
(Date added
to collection) |
Suppliers
Isolate Designation |
Original sample material,
Adapted
to which cell types
(Passage history of sample)
[IAH passage history] |
* Original sample supplied to the collection by person / institution
[IAH, ISIS sample number] |
Comments |
References & accession numbers
|
|
Reference and
vaccine strains |
|
RSArrrr/02
(41010DEC) |
BTV-2
(western topotype
- reference
strain) |
Supplied by OVI
S. Africa to IAH Pirbright
8th Apr 1982 |
NK
|
shelf B2,
box B2γ,
rows C1-D10
&
shelf C2,
box C2γ,
rows C1-D10
Added to
collection on
4th Nov 2005
|
Ref. Strain
Strain:22/59H |
BHK cells
(E2/BHK3)
[IAH/BHK4] |
Duplicate samples
C1(g) supplied on 19th Dec 2007, to JongChul, Jeong Ja Yeon Agrotech Co., Ltd., 385-6,
ChonChon Dong,JangAn-Gu, SuWon-City, KyungKi-Do, 440-330, Korea
*
Duplicate samples, box C2γ- position D2(p)
supplied
to
Oscar R. González Llamazares
(Avenida
Párroco Pablo Díez 49-57, 24010 - Leon - Spain,
22nd Nov 2007)
*
Duplicate samples (Position C2g)
Given to S. Maan
(IAH Pirbright) 12th Aug 2008
*
Vial D2 (p) A&B sent to Mehdi El Harrak, Biopharma
, Morocco 29th June 2009
*
Vial C3 sent to
Denis Kolbasov, Russian National
Institute of Veterinary Virology 29th
Sept 2009
*
Vial C sent to Dr Graham Abdeljelil,
Institut Pasteur de Tunis 8th
March 2011
*
» contents
|
[Seg-1:
KP820910,
Seg-2: KP821030,
Seg-3:
KP821152,
Seg-4:
KP821272,
Seg-5:
KP821392,
Seg-6:
KP821512,
Seg-7:
KP821634,
Seg-8:
KP821754,
Seg-9:
KP821944,
Seg-10:
KP821995
Nomikou et
al 2015 (1)]
[S2:
AJ585123,
S3:
DQ186793,
S5:
AM773705,
S6:AJ586696]
Maan et al 2007 |
|
RSAvvv2/02 |
BTV
Type 2 |
South Africa Onderstepoort Vaccine strain
|
|
Shelf A1
Box ,
Rows
(31/01/2003) |
|
|
Dr. Annalisa Guercio & Giuseppina Alimena) IZS
Palermo |
tubes
1/10a(g)- &
1/10a(p)-D1 given to Helen
Jones, Arbovirology IAH Pirbright 07/06/2004 |
[S2:
AJ585157
S5:
AM773684] |
|
RSAvvvv/02 |
BTV
Type 2 |
South Africa
Vaccine (Onderstepoort) |
|
Shelf B4,
box B4β
Rows
E1-F10 |
BTV-2 vaccine
strain,
batch no.19
exp.01/12/2005 BL6093S |
[IAH/BHK1] |
|
Duplicate vials,
position E1(g) supplied to Sushila Maan
(IAH Pirbright) on 29th April 2009, for sequencing |
» contents
[S6:AJ586665
] |
|
2011 |
|
ISR2011/03 |
BTV-2
Identified as BTV-2
|
Israel
2011 |
|
Shelf C16,
box C16 gamma,
rows I1 - J10
|
|
|
|
|
|
ISR2011/02
|
BTV-2 Identified as BTV-2 by Seg-2
specific
Real time RT-PCR
|
Israel
Afikim,
August 2011
|
Cattle
Blood
Isolated through in eggs at Kimron
|
Shelf C16,
box C16 beta,
rows I1 - J10
Added
|
KVI Id 115249
Lab no: 1822
|
[IAH/KC1]
|
Jacob Brenner
Kimron Institute Israel
Samples arrived 3rd
November 2011
IAH Stars
number
A70/11
02
|
Vials I10 removed from freezer A & B, vial J10 was removed from freezer A. |
|
2010 |
|
ISR2010/34 |
BTV-2 |
collected
in 2010 |
|
Shelf C17,
box C17 Beta,
rows G1-H10 |
|
|
|
|
|
ISR2010/33 |
BTV-2 |
collected
in 2010 |
|
Shelf C17,
box C17 Beta, rows E1-F10 |
|
|
|
|
|
ISR2010/32 |
BTV-2 |
collected
in 2010 |
|
Shelf C17,
box C17 Alpha, rows I1-J10 |
|
|
|
|
|
ISR2010/31 |
BTV-2 |
collected
in 2010 |
|
Shelf C16,
box C16 Gamma, rows G1-H10 |
|
|
|
|
|
ISR2010/30 |
BTV-2
Identified as BTV-2 by Seg-2
specific
Real time RT-PCR
|
Israel,
Elishema
28th
November
2010 |
Isolated from cattle blood
at TPI |
Shelf C15,
box
C15
Gamma,
rows A1-B10
Added
4/10/11 |
3121
KVI Id 90616 |
[KC1 at IAH-P]
|
Jacob Brenne,
Kimron Institute Israel
Samples arrived
30th August 2011
IAH Stars number
A59/11
05 |
|
|
ISR2010/29 |
BTV-2
Identified as BTV-2 by Seg-2
specific
Real time RT-PCR
|
Israel
Zahal
30th
November
2010 |
Isolated from
Eland spleen, at TPI |
Shelf C15,
box
C15
β,
rows I1-J10
Added 4/10/11 |
3134
KVI Id 90888 |
[KC1 at IAH-P] |
Jacob Brenner
Kimron
Institute Israel Samples arrived 30th August 2011
IAH
Stars
number
A59/11
04 |
|
|
ISR2010/28 |
BTV-2
Identified as BTV-2 by Seg-2
specific
Real time RT-PCR
|
Israel
Bet
Gamliel
20th
September
2010 |
Isolated from cattle
blood at IAH-P |
Shelf C15,
box
C15
β,
rows G1-H10
Added 4/10/11 |
2208
KVI Id 84336 |
[KC1 at IAH-P] |
Jacob Brenner
Kimron Institute Israel
Samples arrived
30th
August 2011
|
|
|
ISR2010/24 |
* BTV-2
Identified as BTV-2 by Seg-2 specific
Real
time
RT-PCR
|
Israel |
Isolated from cattle blood at IAH-P |
Shelf C14,
box C14
β,
rows E1-F10
Added to collection
5th July 2011 |
Calf purchased for KVI
870 KVI Id
|
[IAH/KC1]
|
** Jacob Brenner
Kimron Institute Israel
Samples arrived 6TH April
2011
IAH Stars No.
A32/11 6
|
* Note: Originally blood was typed as BTV-8
**Jacob confirmed BTV-2 was in the area in 2004,
many samples could be from mixed infections
Vials F1 were removed from freeezers A & B
|
|
2008 |
|
AUS2008/01 |
BTV-2 |
Australia |
|
Shelf D12,
box D12 α,
rows I1 to J10, |
tube
labelled:
BTV-2 - #0912-17-0002 |
[IAH/BHK1] |
AAHL (BT/2010/145) |
|
Distribution restricted by MTA |
|
2006 |
|
USA2006/04 |
BTV-2
(typed
by
Seg-2 RT-PCR
and sequencing) |
Hillsborough County, FL,
USA
(sample taken
13th Dec 2006) |
From sheep
(clinical
history: oral lesions, cough, respiratory congestion)
Date of virus
preparation in USA
3rd Jan 2007 |
Shelf E3,
box E3α,
rows G1 – H10
Added to collection on
17th Sept 2007
|
06-473635
(tube
labelled: 473635;
IVD BHK/2;
1-3-07) |
From blood
E1/BHK2
[IAH/ BHK4] |
virus isolate
supplied by
USDA, Aphis,
VS, NVSL, PAS
Reagents
Office.
PO box 844
Ames .IA 50010
USA |
Technical
contact in USA:
Donna Johnson
Equine and
Ovine Viruses Section, Diagnostic virus Lab, PO box 844,
Ames IA, 50010, USA.
|
|
2005 |
|
MAR2005/01 |
BTV-2 |
Martinique (France)
(Sample taken Dec 2005) |
from a
non-vaccinated bovine |
Shelf C7,
box C7
α
,
rows C1-D10
(Added to collection
9th Aug 2010)
|
tube
labelled:
BTV-2/06.01 - 108 |
[IAH/BHK1] |
Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France
23rd July 2010 |
|
Distribution restricted by MTA
[S2:] |
|
SPA2005/01 |
Identified as
BTV by RT-PCR and
typed as BTV-2 by
Seg-2 type
specific RT-PCR and sequence analysis |
Spain
blood sample
taken on
11th Jul 2005 |
Virus isolated from cattle blood sample
at IAH Pirbright by Sue Graham |
Shelf C3,
box C3γ.
rows A1-B10
(added to
collection
25th Nov 2005)
|
blood sample
number
4077-23 |
[IAH/E1/BHK2] |
Miguel Angel Jiménez-Clavero
Departamento de Enfermedades Emergentes
[R10/05] |
*
Sample C3 from both freezers given to K. Nomikou
(IAH) , for sequencing 7th July 2011
*
[S2:
AM773697, S5:
AM773685]
OIE report -14th Oct 2005 |
|
2003 |
|
USA2003/08 |
BTV-2
Identified as
BTV-2 by
RT-PCR and
sequence analysis of
Seg-2 |
Mantee County
Florida
USA
Sample taken
21st Oct 2003 |
Virus isolated from
Cattle blood
12th Nov 2003 |
Shelf A16
Box A16
gamma,
Rows H1-I10
(added to
collection on 15th June 2007) |
04-280559-6
tube No.
280559-6;
IVD BHK/1;
11-12-03 |
from blood
ECE1/BHK1
[IAH/KC2]
Related
to isolate USA2003/01 |
virus isolate
supplied by
USDA, Aphis,
VS, NVSL, PAS
Reagents
Office.
PO box 844
Ames .IA 50010
USA |
[S2: ]
Technical
contact in USA: Donna Johnson
Equine and
Ovine Viruses Section, Diagnostic virus Lab, PO box 844
Ames IA, 50010
USA.
Note multiple
serotypes of BTV were isolated from the same herd: BTv-2, 3, 10 and
isolates of unknown serotype. |
|
USA2003/01 |
BTV-2
Identified as
BTV-2 by
RT-PCR and
sequence analysis of
Seg-2 |
Mantee County
Florida
USA
Sample taken
21st Oct 2003 |
Virus isolated from
Cattle blood
12th Nov 2003 |
Shelf E1,
box E1β,
rows A1 - B10
(added to
collection on 15th June 2007) |
04-280559-6
tube No.
280559-6;
IVD BHK/1;
11-12-03 |
from blood
ECE1/BHK1
[IAH/BHK3]
Related to USA2003/08 |
virus isolate
supplied by
USDA, Aphis,
VS, NVSL, PAS
Reagents
Office.
PO box 844
Ames .IA 50010
USA |
[S2: ]
Technical
contact in USA: Donna Johnson
Equine and
Ovine Viruses Section, Diagnostic virus Lab, PO box 844
Ames IA, 50010
USA.
Note multiple
serotypes of BTV were isolated from the same herd: BTv-2, 3, 10 and
isolates of unknown serotype. |
|
IND2003/03 |
BTV
Type 2
(Typed by
SNT & by Seg-6 RT-PCR / sequence analysis) |
Tiruneveli,
Tamil Nadu,
India |
Sheep
(Isolated at Tamil Nadu Veterinary And Animal Sciences
University, Chennai-600 051 (India)) |
Shelf A6
Box A6β
Rows E1-F10 |
(138) |
BHK cells
(BHK5)
[IAH/E1/BHK3] |
Dr A. Wilson
Aruni |
Tube F1 (freezer
A&B) given to
Kiki Nomikou, Athens Vet lab, Greece, Oct 2004
*
Tube F2 given to Sushila
Maan IAH Pirbright 04th May 2006
*
Tube E10
removed from freezer A&B
*
[Seg6:AJ783906] (MTA) |
|
IND2003/02 |
BTV
Type 2
(Typed by
SNT & by Seg-6 RT-PCR / sequence analysis)
|
Tiruneveli,
Tamil Nadu,
India |
Sheep
(Isolated at Tamil Nadu Veterinary And Animal Sciences
University, Chennai-600 051 (India)) |
Shelf B6,
Box B6α
Rows E1-F10
(added to
collection on
23 Aug 2005)
|
(132) |
BHK cells
(BHK5)
[IAH/E1/BHK2] |
Dr A. Wilson
Aruni |
sample 1/10g given to Sushila
Maan IAH Pirbright
04th May 2006 |
[Seg6:AJ783905]
(MTA) |
|
IND2003/01 |
BTV
Type 2
(Typed by
SNT & by Seg-6 RT-PCR / sequence analysis)
|
Tiruneveli,
Tamil Nadu,
India |
Sheep
(Isolated at Tamil Nadu Veterinary And Animal Sciences
University, Chennai-600 051 (India)) |
Shelf B5,
box B5γ,
rows G1- H10
(added to collection on
16/08/2005)
|
(102) |
BHK cells
(BHK5)
[E1/BHK3] |
Dr A. Wilson
Aruni |
Sample 1/10g given to Sushila
Maan IAH Pirbright
04th May 2006
----------------- Duplicate
samples
G1(g) supplied on 19th Dec
2007, to JongChul, Jeong
Ja Yeon Agrotech Co., Ltd.,
385-6, ChonChon-Dong, JangAn-Gu, SuWon-City, KyungKi-Do, 440-330, Korea
|
[Seg6:AJ783904]
(MTA) |
|
ZIM2003/01
|
BTV
Type 2
(Typed by
Rt-PCR)
|
Zimbabwe
(Lone Oak)
(25/08/03
) |
Cuilcoides
imicola |
Shelf A4,
Box A4β,
Positions
G1-H10 |
|
BHK cells
(E1/BHK2) |
[R6/03 No. 92]
|
Tube G10
freezer A and B
given to Sushila
Maan IAH Pirbright 04th May 2006
*
Vial position H10 was
removed from freezer A
and B
29th Mar 2012,
*
Vial position G9 from Freezer A given to Elizabeth
Morecroft (Pirbright) 9th Jul 2013.
*
Tube H9 was removed from the collection
*
[S2:
] |
|
2002 |
|
ITL2002/07
|
BTV
Type 2
(Typed by
SNT & by Seg-2 RT-PCR / sequence analysis) |
Sicily
S.Angelo di Brolo (ME)
Farm code:
088ME036 E 14o 54432 N 38o 04925 Alt. 650 (18/Jul/2002) |
From Sheep
(spleen)
(isolated at:
IZS Palermo by: Dr. Annalisa Guercio & Giuseppina Alimena) |
Shelf A1,
Box A1γ,
Positions
E1-F10
(31/Jan/2003) |
(32990/A010)
|
BHK cells
(E1, BHK3)
[IAH-BHK4]
|
Dr. Annalisa
Guercio & Giuseppina Alimena) IZS Palermo
Isolated from
the same location (same farm) as ITL2002/05 |
*
Tubes E1 & F1 from freezer A supplied to S. Anthony & H. Jones, Pirbright Institute
7th June 2004
*
Vial position E2 Freezer A given to C. Batten (IAH Pirbright) 21 Sept 2008 for ring trial
spare vial from freezer B
held in misc box
*
Sample E3 from freezer A given to K. Nomikou
(IAH) , for sequencing 7th July 2011
* [S2:AJ585160,
S3:DQ186815,
S5:
AM773688
S6:AJ586671] |
|
ITL2002/06
|
BTV
Type 2
(Typed by SNT &
by Seg-2 RT-PCR + sequence analysis) |
Sicily
Raccuja (ME)
Farm code: 069ME006 E 14o 55664 N 38o 01318 Alt. 493
(06/Aug/2002) |
From a pool of
348 Culicoides pulicaris
(isolated at:
IZS Palermo by: Dr. Annalisa Guercio & Giuseppina Alimena)
|
Shelf A1,
Box A1β,
Positions
E1-F10
(31/Jan/2003) |
(36183)
|
BHK cells
(E1, BHK3)
[IAH-BHK1] |
Dr. Annalisa
Guercio & Giuseppina Alimena) IZS Palermo
Note:
TL2002/ 01
to ITL2002/06
Represent some of the first
field isolates from C. Pulicaris
|
Tubes E1 & F1 from freezer A
supplied to S. Anthony
& H. Jones, Pirbright Institute
7th June 2004
[S2:
S6:AJ586670, S10:
] |
|
ITL2002/05
|
BTV Type 2
(Typed by SNT & by Seg-2 RT-PCR
+ sequence analysis)
|
Sicily
S.Angelo di Brolo (ME) Farm code: 088ME036 E 14o 54432 N 38o 04925
Alt. 650
(06/Aug/2002) |
from a pool of
122 Culicoides pulicaris (At: IZS Palermo by: Dr. Annalisa Guercio &
Giuseppina Alimena) |
Shelf A1,
Box
A1β, positions
C1-D10
(31/Jan/2003) |
(36179)
|
BHK cells
(E1, BHK3)
[IAH-BHK1]
|
Dr. Annalisa
Guercio & Giuseppina Alimena) IZS Palermo Isolates
Isolated from
the same location (same farm) as ITL02/07 |
Tubes C1+2 & D1+2 from freezer A supplied to S. Anthony &
H. Jones,
Pirbright Institute
7th June 2004
D3 from freezer A also removed from collection
» contents
[S6:AJ586669
] |
|
ITL2002/04
|
BTV
Type 2
(Typed by
SNT & confirmed by Seg 2 RT-PCR) |
Sicily S.
Pietro Patti (ME) Farm code: 081ME039 E 14o 975933 N 38o 0079 Alt.1080
(06/Aug/ 2002) |
from a pool of
156 Culicoides pulicaris
(isolated at:
IZS Palermo by: Dr. Annalisa Guercio & Giuseppina Alimena) |
Shelf A1,
Box
A1β,
positions
A1-B10
(31/Jan/2003) |
(36178) |
BHK cells
(E1, BHK3)
[IAH-BHK1]
|
Dr. Annalisa
Guercio &
Giuseppina
Alimena
IZS Palermo, Sicily
Note:
TL2002/ 01
to ITL2002/06
Represent some of the first
field isolates from C. Pulicaris |
Tubes A1 & B1 from freezer A
supplied to S. Anthony &
H. Jones, Pirbright Institute
7th June 2004
[S10: ] |
|
ITL2002/03
|
BTV Type 2
(Typed by SNT and confirmed by Seg 2 RT-PCR) |
Sicily
Novara Di
Sicila (ME)
Farm code:
062ME005 E 15o 06352 N38o 00130 Alt. 491
(24 July
2002) |
from a
pool of 200 Culicoides pulicaris
(Isolated at:
IZS Palermo by: Dr. Annalisa Guercio & Giuseppina Alimena) |
Shelf A1,
Box
A1α,
Positions
I1-J10 (31/Jan/2003) |
(34125) |
BHK cells
(E1, BHK3)
[IAH-BHK1]
|
Dr. Annalisa
Guercio &
Giuseppina Alimena
IZS Palermo, Sicily |
Tubes I1 & J1
from freezer A
supplied to S. Anthony &
H. Jones, Pirbright Institute 7th June 2004
*
» contents
[S10:
] |
|
ITL2002/02 |
BTV
Type 2
(Typed by SNT,
Type confirmed by Seg 2 RT-PCR) |
Sicily
Novara Di Sicila
(ME)
Farm code
062ME015 Grid reference E 15o 07089 N 38o 00421 Alt 575
(18th July
2002) |
from a
pool of 18 Culicoides pulicaris
(Isolated At:
IZS Palermo by: Dr. Annalisa Guercio & Giuseppina Alimena) |
Shelf A1,
Box
A1α,
Positions
G1-H10
(31/Jan/2003) |
(33669) |
BHK
cells
(E1, BHK5) |
Dr. Annalisa
Guercio &
Giuseppina Alimena
IZS Palermo, Sicily |
* Tubes G1and H1
from freezer A supplied to
S. Anthony and H.
Jones, Pirbright
Institute
7th June 2004
* |
|
ITL2002/01 |
BTV
Type 2
(Typed by SNT &
confirmed by Seg
2
RT-PCR) |
Sicily
Novara Di Sicila
(ME) Farm code 062ME015 Grid reference E 15o 07089 N 38o 00421 Alt 575
(18/
Jul/2002) |
from a pool of
18 Culicoides pulicaris
(Isolated at:
IZS Palermo by: Dr. Annalisa Guercio & Giuseppina Alimena) |
Shelf A1,
Box
A1α, Positions
E1-F10
(31/Jan/2003) |
(33669) |
BHK
cells
(E1, BHK3)
[IAH-BHK1] |
Dr. Annalisa
Guercio &
Giuseppina Alimena
IZS Palermo, Sicily |
Tubes A1+2
&
F1+2 from freezer A supplied to
S. Anthony and
H. Jones, IAH Pirbright
7th July 2004
» contents
[S2:AM773708
S5:AM773687 ]
|
|
SAD2002/03 |
BTV Type 2 |
Sardinia
(02/01/02) |
Sheep |
Shelf A6,
Box A6γ
Rows C1-D10 |
(B4) |
BHK cells
(E1/BHK2)
[IAH-BHK1] |
|
aliquots D1
(freezer A&B) Sent to Kiki Nomikou, Athens Vet Labs , Greece, Oct 2004
[S10: ] |
|
SAD2002/02 |
BTV Type 2 |
Sardinia
Isolated at IAH from Sheep blood collected on
2nd January 2002)
|
Sheep |
Shelf D5
box D5α,
rows I1-J10,
Added to
the collection on the 2nd Feb 2007 |
(13 P) |
BHK cells
(IAH/E1/BHK2) |
|
*
Vial positions
E1 (p)
were given to Eva
Veronesi (IAH Pirbright on 13th May 2010 for Ph.D studies).
one of these was retained in Misc
box C, bottom of freezer A
position E5
*
Duplicate samples E3 given to K. Nomikou (IAH)
for sequencing 7th July 2011
* |
|
SAD2002/01 |
BTV Type 2 |
Sardinia
(02/01/02) |
Sheep |
Shelf D1,
Box D1ß,
Rows G1 to H 12
(Added to
collection on
8th Aug 2006)
|
(10 P) |
Adapted to BHK cells
E1/BHK2
+
[IAH/BHK1] |
|
[S10: ]
*
Duplicate sample from position
position A1p sent to John Murray / Jeremy Salt
Moredun Scientific Scotland 6th Sept 2010.
*
|
|
2001 |
|
ISR2001/17
|
BTV
Type 2
yped by
Seg-2 RT-PCR
and sequence comparisons |
Israel, Yokneam |
Sheep (27/Feb/2001
at the Kimron Institute) |
Shelf B6,
Box B6β
Rows I1-J10
(Added to
collection on
1st Oct 2005)
|
(300180
Lab No:823/2)
|
(E1)
[IAH/BHK5] |
Dr. Hagai Yadin,
Kimron
Institute Israel |
(MTA) |
»
contents |
|
FRA2001/07 |
BTV
Type 2
|
Corsica
2nd Sept 2001 |
Sheep
Isolated at TPI from spleen |
Shelf
D12,
box
D12 alpha,
rows G1-H10
(Added to
collection on
23rd July 2014)
|
[R16/ 01 - 21] |
BHK cells
[E1/BHK4]
|
Supplied by Stephan
Zientara, AFSSA, Maisons Alfort France September 2001
|
|
|
FRA2001/06 |
BTV
Type 2 |
Corsica
2nd Sept 2001 |
Sheep |
Shelf A1,
Box
A1γ,
Rows G1-H10 |
[R16/ 23] |
BHK cells
(E1/BHK2) |
|
*
Tubes
G1 & H1 Freezer A supplied to S. Anthony & H. Jones,
IAH Pirbright 07-06-2004
*
Tubes position G2
Freezer
A given to C. Batten (IAH-P)
28th April 2010 for
Ring Trial
*
» contents |
|
FRA2001/05 |
BTV Type 2 |
Corsica
2nd Sept 2001
|
Sheep
Isolated at TPI from spleen
|
Shelf
D12,
box
D12β,
rows
E1-F10
(Added
to
collection on
23rd July 2014)
|
[R16/ 14] |
BHK cells
[E1/BHK3] |
Supplied by Stephan Zientara, AFSSA,
Maisons Alfort France September 2001 |
[S10: ] |
|
FRA2001/04 |
BTV Type 2 |
Corsica
(12/09/01) |
Sheep |
Shelf B5,
Box B5β,
Rows I1 - J10
(added to collection on
1st Aug 2005)
|
[R16/ 12] |
BHK cells
(E1/BHK2)
[IAH/BHK2] |
|
|
» contents
|
|
FRA2001/03 |
BTV
Type 2
(typing
confirmed by Seg 2 RT-PCR and sequencing) |
Corsica
(12/09/01) |
Sheep
|
Shelf B1,
Box B1α
Rows C1-D10
|
[R16/ S2] |
BHK cells
(BHK2)
[IAH/BHK1] |
|
[S2:
AJ585121,
S5:
AM773686,
S6:AJ586674]
*
Sample D2 from both freezers given to K. Nomikou
(IAH) , for sequencing on 7th July 2011
* |
|
FRA2001/02 |
BTV
Type 2
|
Corsica
(12/09/01) |
Sheep
Isolated at AFSSA-
Maisons Alfort France
September
2001
|
Shelf D12,
Box D12β,
rows C1-D10
(Added to
collection
on
23rd July 2014) |
[R16/ S1] |
BHK cells
E1/BHK1[BHK3] |
Supplied by Stephan Zientara, AFSSA, Maisons Alfort France September
2001 as BHK cell culture material |
» contents
|
|
FRA2001/01 |
BTV
Type 2 |
Corsica
(31/07/01) |
NK |
shelf C5
box C5α
rows E1-F10
(Added to
collection on
22 Dec 2005) |
[R10/01; BT44] |
BHK cells
(V1/BHK3)
[IAH/BHK2] |
|
[S10:
]] |
|
SAD2001/08 |
BTV Type 2 |
From Sardinia
Isolated at IAH Pirbright
(Sample taken 29th Dec 2001) |
Sheep |
Shelf C15,
box C15γ,
rows I1-J10
Added to
collection on
14 Oct 2011 |
(4P) |
BHK cells
[IAH/E1/BHK1/KC1] |
|
Derived from
SAD2001/01
*
100ml of this passage level sent to Michael Eschbaumer at FLI
Rheims Germany , as a vaccine challenge strain on 13Oct 2011 under
MTA
*
100ml of this passage level sent to Piet van Rijn
CVI at Lelystad the Netherlands, as a vaccine challenge strain under
MTA
* |
|
SAD2001/07 |
BTV Type 2 |
Sardinia
(29/12/01) |
isolated from Sheep |
Shelf C4,
box C4α
rows A1-B10
(Added to
collection on
21st Dec 2005) |
(FF7) |
Adapted to BHK cells
E1/BHK2
[IAH/BHK2] |
|
|
[S10:
] |
|
SAD2001/06 |
BTV Type 2 |
Sardinia
(29/12/01) |
Sheep |
Shelf C4,
box C4β
rows I1 - J10
(added to
collection
19th Dec 2005) |
(FF4) |
adapted to
BHK cells
E1/BHK2
[IAH/BHK2] |
|
|
» contents
[S10: ] |
|
SAD2001/05 |
BTV Type 2 |
Sardinia
(31/12/01) |
Sheep |
Shelf C4,
box C4β
rows G1 - H10
(added to
collection
19th Dec 2005) |
(17 P) |
adapted to
BHK cells
E1/BHK2
[IAH/BHK2] |
|
|
» contents
[S10: ] |
|
SAD2001/04 |
BTV Type 2 |
Sardinia
(29/12/01) |
Sheep |
Shelf C4,
box C4β
rows E1 - F10
(added to
collection
19th Dec 2005) |
(12 P) |
adapted to
BHK cells
E1/BHK2
[IAH/BHK2] |
|
|
» contents
[S10: ] |
|
SAD2001/03 |
BTV Type 2 |
Sardinia
(29/12/01) |
Sheep |
Shelf A6,
Box A6γ
Rows E1-F10 |
(7 P) |
BHK cells
(E1/BHK2)
[IAH-BHK1] |
|
aliquots F1
(freezer A&B) sent to Kiki Nomikou, Athens Vet Labs , Greece, Oct 2004
» contents
[S10: ] |
|
SAD2001/02 |
BTV Type 2 |
Sardinia
(29/12/01) |
Sheep |
Shelf C4,
box C4β
rows C1 - D10
(added to
collection
19th Dec 2005)
|
(6P) |
adapted to BHK cells
E1/BHK2
[IAH/BHK2] |
|
|
[S2:
AJ585162
S5:
AM773706
S6:AJ586673] |
|
SAD2001/01 |
BTV Type 2 |
From Sardinia
Isolated at IAH Pirbright
(Sample taken 29th Dec 2001) |
Sheep |
Shelf D6,
box D6β,
rows A1-B10
Added to
collection on 2nd Feb 2007 |
(4P) |
BHK cells
[IAH/E1/BHK2] |
|
» contents
*
Duplicate vials position A1 (P), taken out of freezers A and B on 6th Sept
2010
*
Used to generate SAD2001/08 as vaccine challenge
strain
*
Duplicate vials position A4 from freezers A and B
given to K. Nomikou (3rd May 2012) to prepare
SAD2001/08,
*
Vial position B2 (G) taken out of
freezer A and B 29th Mar
2012
*
[S6:AJ586672] |
|
2000 |
|
TUN2000/02 |
BTV Type 2 |
Tunisia |
NK |
|
[R2/00; BT 74] |
|
|
|
|
TUN2000/01 |
BTV Type 2 |
Tunisia
(3rd Feb 2000) |
NK |
shelf B5,
box B5γ,
rows A1-B10
(added to collection on 16/08/2005) |
[R1/00 ;
BT 74] |
BHK cells
(E1/BHK2)
[IAH/BHK3] |
|
|
» contents
[Seg2:AJ585156
Seg3:DQ186826
Seg5:AM773689
Seg6:AJ586668]
|
|
1994 |
|
IND1994/01 |
BTV-2 |
South India
|
|
Shelf C16,
box C16α,
rows E1-F10
Added to collection
2006 |
|
Unknown
passage
level in
BHK cells
[IAH/
BHK2] |
|
Sample given to Sushila Maan (IAH Pirbright) Sept 2006 for
sequencing studies
(MTA) |
|
1993 |
|
IND1993/01 |
BTV-2
Typed by real-time RT-PCR targeting Seg-2 |
Southern India
(1993) |
From sheep |
Shelf C16,
box C16α
rows A1-B10
Added to collection 2006
|
|
Unknown passage level in BHK cells
[IAH/BHK2] |
|
Sample given to Sushila Maan
IAH Pirbright Sept 2006 for sequencing studies
*
(MTA) |
|
1985 |
|
SUD1985/01 |
BTV
Type 2 |
Sudan (15/01/85)
|
NK |
Shelf C4,
box C4α
rows C1-D10
(Added to
collection on
21st Dec 2005) |
(GP-4; SVD9/83) |
adapted to BHK cells
LD3/BHK3
[IAH/BHK2] |
|
|
[S2:
S6:AJ586666] |
|
1983 |
SUD1983/07
(SUD----/03) |
BTV-2
|
Sudan
Nyala
(western Sudan)
|
Isolated
at IAH Pirbright, from a pool
of Culicoides
collected in Sept/Oct 1983
|
Shelf B11,
box
γ,
rows C1-D10
Added to collection
22nd Oct 2009
|
GP14 |
[LD3/BHK5/
PP2/BHK1**]
|
M.E.H. Mohammed |
|
|
SUD1983/05
(SUD----/01) |
BTV-2
|
Sudan
Nyala
(western Sudan)
|
Isolated at IAH Pirbright, from a pool
of Culicoides imicola
collected in Sept/Oct 1983 |
Shelf B11,
box γ ,
rows A1-B10
Added to collection
22nd Oct 2009 |
GP4 |
[LD3/BHK5**] |
M.E.H. Mohammed |
Vial Position A2 was from freezer A was givemn to
Kyriaki Nomikou (Pirbright) on 14th February 2013
Equivalent vial in Freezer B
was not removed |
|
1982 |
|
IND1982/01 |
BTV Type 2 |
India
(26/03/82) |
NK |
shelf C2,
box C2β,
rows G1-H10
Added to collection on
25th Oct 2005 |
(IND - 171) |
BHK cells
(E2/BHK2 )
[IAH/BHK1] |
|
Sample Nos. 1/10g(a+b) were given to
Natalie Ross-Smith (IAH Pirbright) for sequencing studies,
7th March
2006 |
[S2:
AJ585152, S6:AJ586675]
(MTA) |
NIG1982/02
|
BTV Type 2
(typing
confirmed by Seg 2 RT-PCR and sequencing) |
Nigeria
(05/09/82) |
NK |
shelf C5
box C5α
rows A1-B10
(Added to
collection on
22nd Dec 2005) |
( N –350) |
BHK cells
(E1/BHK3)
[IAH/BHK2] |
|
|
» contents
[S2:
S6:AJ586667] |
|
1971 |
|
RSA1971/07 |
BTV-2
(see BTV2
RSA1971/03) |
Grown at IAH in sheep from a
freeze dried blood sample of
RSA1971/03. Originally
sampled from the field
between
1968-1972 |
Sheep, D8 post infection, sheep clinically ill |
26th April 2014 |
BTV2
RSA1971/03 |
Original sample:
Freeze dried blood from sheep infected with BTV-2 RSA1971/03 |
This sample is derived from
RSA1971/03
(IAH/5 x sheep and
2 x egg) |
|
|
|
RSA1971/03 |
BTV-2
confirmed by
serotype specific RT-PCR targeting genome segment 2 |
Grown at IAH in
Dorset Poll sheep from a sample of virus originally isolated
between
1968-1972 |
|
|
(3032) |
no passage in
cell culture |
This virus has
been passed 4 times in sheep.
It has been grown in Eggs and
the last two sheep passes were infected with freeze dried blood plus
infected egg material
|
Freeze dried Virus in sheep
blood
(Dorset Poll) |
|
