|
Family:
Reoviridae, Genus: Orbivirus, Species:
Bluetongue virus (BTV)
serotype 9 |
|
*IAH dsRNA virus collection number
( ICTVdb
isolate Accession Number) |
Virus Species
&
Serotype
(identification method) |
Place of original sample eg country town
[grid reference]
(date original sample was taken) |
Isolated from which host species e.g cow, sheep, etc
(Date of virus isolation, where, by whom) |
Position of storage in reference freezers
(Date added to collection) |
Suppliers
Isolate Designation |
Original sample material,
Adapted to which cell types
(Passage history of sample)
[IAH passage history] |
* Original sample supplied to the collection by person / institution
[IAH, ISIS sample number]
Comments |
References & accession numbers
|
|
Reference and vaccine strains |
|
RSArrrr/09
(41010EDA) |
BTV - 9
(reference
strain - 'western' topotype) |
|
NK |
Shelf B3,
box B3α
rows G1-H10
Added to collection on 9th May 2005
|
(Reference
Strain) |
(E3/BHK6)
+
[IAH/BHK2] |
Supplied by OVI
S. Africa (recieved 22/12/82)
*
Duplicate
samples G1(g) supplied on 19th Dec 2007, to JongChul, Jeong Ja
Yeon Agrotech Co., Ltd., 385-6, ChonChon-Dong, JangAn-Gu, SuWon-City,
KyungKi-Do, 440-330, Korea
* Vial H1 (p) A&B sent to Mehdi
El Harrak, Biopharma , Morocco 29th June 2009
* Vial G3(g) A+B -
given to Kyriaki Nomikou (IAH Pirbright) 2nd March 2010
*
Vial H2 sent to
Denis Kolbasov, Russian National
Institute of Veterinary Virology 29th
Sept 2009
*
Vial H sent to Dr Graham Abdeljelil,
Institut Pasteur de Tunis 8th
March 2011
*
|
[Seg-1:
KP820968,
Seg-2: KP821088,
Seg-3: KP821210,
Seg-4: KP821330,
Seg-5: KP821450,
Seg-6: KP821570,
Seg-7: KP821692,
Seg-8: KP821812,
Seg-9: KP821932,
Seg-10: KP822053,
Nomikou et al 2015 (4)]
[S2:AJ585130,
S3:
DQ186795,
S6:AJ586708]
Maan et al 2007 »
contents |
|
ITLvvv2/09 |
BTV
Type 9 |
BTV-9 Vaccine
Strain
|
|
|
|
(data not available) [BHK1] |
Sample
Derived from
ITLvvv1/09, but
passaged at IAH in BHK cells |
|
|
ITLvvv1/09
|
BTV
Type 9 |
BTV-9 Vaccine
produced in Italy
(IZS
Terramo)
batch number 006, expiry
date date 06/04. |
|
Shelf D5,
box D5β,
rows I1-J10
Added to
collection
9th
Feb 2007 |
BTV 9 vaccine
batch number 006, expiry
date date 06/04. |
(data not available) |
freeze dried monovalent
commercial vaccine.
(used to generate
ITLvvv2/09) |
»
contents |
|
RSAvvv1/09 |
BTV
Type 9
(Type confirmed by:
Seg 2 RT-PCR and
sequence analysis) |
S.Africa
14/04/02
|
NK |
Shelf A4,
Box A4β,
Rows A1-B10 |
Vaccine strain
[R8/02, No 1] |
BHK
(???)
[IAH- BHK2] |
Sue Graham (June
2002)
|
[S5:
AM778442] |
|
RSAvvv2/09 |
BTV
Type 9
(Type confirmed by:
Seg 2 RT-PCR and
sequence analysis) |
S. Africa
Onderstepoort
Vaccine |
|
Shelf A1,
Box A1α,
Rows
C1-D10
Added to
collection on
31st Jan 2002 |
|
[BHK1] |
Dr. Annalisa
Guercio
IZS Palermo |
Tubes C1 &D1
freezer A
sent to S. Anthony & H. Jones,
Pirbright
Institute 7th
June 2004 »
contents
[S2:AJ585173, S6:AJ586683
S8:AM900374
S9:
EU220295] |
|
RSAvvv3/09 |
BTV
Type 9 |
S. Africa
Onderstepoort Vaccine |
|
Shelf B6,
box B6γ,
rows E1-F10
Added to
collection
7th Sept 2005
|
Commercial
Vaccine
Batch no.2
exp. 01/11/2005
BL6094S
|
(NK)
[IAH-BHK1] |
Dr. Annalisa
Guercio IZS Palermo |
|
|
|
RSAvvv9/09 |
BTV
Type 9 |
S.Africa
14/04/02
|
NK |
|
Vaccine strain
[R8/02 No 9] |
BHK
( )
|
Sue Graham (June
2002)
|
|
|
|
2008 |
|
LIB2008/10 |
BTV-9
Identified by
BTV group-specific
RT-PCR
[2]
Typed by
BTV-9 specific
real-time PCR |
Libya |
Blood from Sheep with
clinical signs of BT
(Field sample) |
Shelf B10,
box B10 b,
rows E1-F10
Added to collection
18th Sept 2009
|
Animal No C6 |
[IAH/KC1/BHK1] |
From :
Jimmy Elaref
Received on approximately
14th Oct 2008
[A160/08 17] |
Isolate derived from
LIB2008/07 |
|
|
LIB2008/09 |
BTV-9
Identified by
BTV group-specific
RT-PCR
[2]
Typed by
BTV-9 specific
real-time PCR |
Libya |
Blood from Sheep with
clinical signs of BT
(Field sample)
|
Shelf B10,
box B10 b,
rows C1-D10
Added to collection
18th Sept 2009 |
Animal No
C1 |
[IAH/KC1/BHK1] |
From :
Jimmy Elaref
Received on approximately
14th Oct 2008
[A160/08 12] |
Isolate derived from LIB2008/04 |
|
|
LIB2008/08 |
BTV-9
Identified by
BTV group-specific
RT-PCR
[2]
Typed by
BTV-9 specific
real-time PCR |
Libya |
Blood from Sheep with
clinical signs of BT
(Field sample)
|
Shelf B10,
box B10 b,
rows A1-B10
Added to collection
18th Sept 2009 |
Animal No b2 |
[IAH KC1/BHK1] |
From :
Jimmy Elaref
Received on approximately
14th Oct 2008
[A160/08 5] |
Isolate derived from
LIB2008/01 |
|
|
LIB2008/07 |
BTV-9
Identified by
BTV group-specific
RT-PCR
[2]
Typed by
BTV-9 specific
real-time PCR |
Libya |
Blood from Sheep with
clinical signs of BT
(Field sample) |
Shelf B7,
box B7 γ,
rows E1-F10
Added to
collection
23rd
Jan 2009 |
Animal No C6 |
(IAH KC1 ) |
From :
Jimmy Elaref
Received on approximately
14th Oct 2008
[A160/08 17] |
Used to Generate
LIB2008/10 |
|
|
LIB2008/06 |
BTV-9
Identified by
BTV group-specific
RT-PCR
[2]
Typed by
BTV-9 specific
real-time PCR |
Libya |
Blood from Sheep with
clinical signs of BT
(Field sample)
|
Shelf B7,
box B7 γ,
rows C1-D10
Added to
collection
23rd
Jan 2009
|
Animal No
C5 |
(IAH KC2 ) |
From:
Jimmy
Elaref
Received on approximately
14th Oct 2008
[A160/08 16] |
|
|
|
LIB2008/05 |
BTV-9
Identified by
BTV group-specific
RT-PCR
[2]
Typed by
BTV-9 specific
real-time PCR |
Libya |
Blood from Sheep with
clinical signs of BT
(Field sample)
|
Shelf B7,
box B7 γ,
rows A1-B10
Added to
collection
23rd
Jan 2009 |
Animal
No C3 |
(IAH KC1 ) |
From :
Jimmy Elaref
Received on approximately
14th Oct 2008
[A160/08 14] |
|
|
|
LIB2008/04 |
BTV-9
Identified by
BTV group-specific
RT-PCR
[2]
Typed by
BTV-9 specific
real-time PCR |
Libya |
Blood from Sheep with
clinical signs of BT
(Field sample)
|
Shelf B7,
box B7
β,
rows I1-J10
Added to
collection
23rd
Jan 2009 |
Animal No
C1 |
[IAH/KC1] |
From :
Jimmy Elaref
Received on approximately
14th Oct 2008
[A160/08 12] |
Used to generate isolate LIB2008/09 |
|
|
LIB2008/03 |
BTV-9
Identified by
BTV group-specific
RT-PCR
[2]
Typed by
BTV-9 specific
real-time PCR |
Libya |
Blood from Sheep with
clinical signs of BT
(Field sample)
|
Shelf B7,
box B7
β,
rows G1-H10
Added to
collection
23rd
Jan 2009 |
Animal No
Q4 |
(IAH KC1
) |
From :
Jimmy Elaref
Received on approximately
14th Oct 2008
[A160/08 9] |
Vial position G1 (g) from freezer A given to
Kyriaki Nomikou 24-Apr-2012 to prepare RNA to be sent to Sanger Centre
for sequencing.
|
|
LIB2008/02 |
BTV-9
Identified by
BTV group-specific
RT-PCR
[2]
Typed by
BTV-9 specific
real-time PCR |
Libya |
Blood from Sheep with
clinical signs of BT
(Field sample)
|
Shelf B7,
box B7,
β rows E1-F10
Added to
collection
23rd
Jan 2009 |
Animal No Q3 |
(IAH KC1 ) |
From :
Jimmy Elaref
Received on approximately
14th Oct 2008
[A160/08 8] |
|
Vial position F10
(P) taken out of freezer A
and B
on 29th Mar 2012
|
LIB2008/01 |
BTV-9
Identified by
BTV group-specific
RT-PCR
[2]
Typed by
BTV-9 specific
real-time PCR |
Libya |
Blood from Sheep with
clinical signs of BT
(Field sample)
|
Shelf B7,
box B7
β,
rows C1-D10
Added to
collection
23rd
Jan 2009 |
Animal No b2 |
(IAH KC1 ) |
From :
Jimmy Elaref
Received on approximately
14th Oct 2008
[A160/08 5] |
Used to generate
LIB2008/08
*
Vial positions E1 (p)
were given to Eva Veronesi (IAH Pirbright
on 13th May 2010 for Ph.D studies).
Spare vial retained in freezer B position E1.
*
Vial D10 freezer A given to C. Batten (IAH-P)
9th June 2009
Spare vial retained in freezer B position D10
*
Duplicate vials position C2g , taken out of
freezers A and B
on 6th Sept 2010
*
Vial position D2
(P) taken out of freezer A
and B on 29th Mar 2012
|
|
2005 |
|
IND2005/08 |
BTV-9 |
Southern India |
from
sheep
Sample taken in 2005 |
Shelf ??
box ??,
rows ??
(added to
collection
2006) |
|
unknown passage level in BHK cells
[IAH/KC1/BHK1] |
|
Sample given to Sushila Maan IAH Pirbright Sept 2006 for
sequencing studies
*
Derived from IND2005/07
(MTA) |
|
IND2005/07 |
BTV-9 |
Southern India |
from
sheep
Sample taken in 2005 |
Shelf ??
box ??,
rows ??
(added to
collection
2006) |
|
unknown passage level in BHK cells
[IAH/KC1] |
|
Sample given to Sushila Maan IAH Pirbright Sept 2006 for
sequencing studies
*
Used to generate IND2005/08
(MTA) |
|
IND2005/03 |
BTV-9 |
Hyderabad,
India |
|
Shelf D4
box D4 β,
rows A1-B10
(added to
collection
21st
Nov2006) |
|
[+IAH/
BHK3] |
|
Sample given to Sushila Maan IAH Pirbright Sept 2006 for
sequencing studies |
(MTA) |
|
IND2005/02 |
BTV-9 |
Hyderabad,
India |
|
Shelf D4
box D4α,
rows I1-J10
(added to
collection
21st
Nov2006)
|
|
[+IAH/
BHK3] |
|
Sample given to Sushila Maan IAH Pirbright Sept 2006 for
sequencing studies |
[S8:
AM900385]
(MTA) |
|
IND2005/01 |
BTV-9 |
Hyderabad,
India |
Heparinised Blood |
Shelf D4,
box D4α,
rows G1-H10
(added to
collection
21st
Nov2006) |
|
[+IAH/
BHK3] |
|
Sample given to Sushila Maan IAH Pirbright Sept 2006 for
sequencing studies |
(MTA) |
|
MAR2005/06 |
BTV-9 |
Martinique (France)
(Sample taken
Dec 2005) |
from a
non-vaccinated bovine |
Shelf C7,
box C7 α ,
rows A1-B10
(Added to collection
9th Aug 2010) |
tube labelled:
W1B5 xx 130 |
E1BHK5
[IAH/BHK1 ]
|
Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France
23rd July 2010 |
|
Distribution restricted by MTA
[S2: ] |
|
2004 |
|
IND2004/04 |
BTV-9 |
Hyderabad,
India |
Heparinised Blood |
Shelf D4,
box D4α,
rows E1-F10
(added to
collection on 20th Nov 2006)
|
|
[IAH/
BHK3 ] |
|
Sample given to Sushila Maan IAH Pirbright Sept 2006 for
sequencing studies |
(MTA) |
|
IND2004/02 |
BTV-9 |
Andhra
Pradesh
Sample taken
2004 |
From Blood |
Shelf D3
box D3γ,
rows I1-J10
(added to
collection
7th Nov
2006) |
No. 12 |
[IAH/BHK3] |
|
Sample given to Sushila maan IAH Pirbright Sept 2006 for
sequencing studies |
(MTA) |
|
2003 |
|
IND2003/11 |
BTV-9 |
India |
|
Shelf D5
Box D5 ALPHA
Rows C1-D10
(added to
collection on
27th Nov 2006) |
|
[IAH/
BHK3 ] |
|
Sample given to Sushila maan IAH Pirbright Sept 2006 for
sequencing studies
27th Nov 2006 |
(MTA) |
|
ITL2003/01
|
BTV
type 9
(Type confirmed
by:
Seg 2 RT-PCR
and
sequence analysis) |
Sicily Farm code:
33306
Province: Nissoria (Enna)
(2nd Apr.2003) |
Sheep
(04/11/2003) |
Shelf A3,
Box A3γ,
Rows I1-J10
|
(5326/2003) |
from spleen
( BHK4)
[IAH-BHK3] |
Dr. Annalisa
Guercio &
Giuseppina Alimena
IZS Palermo
From Sheep spleen
died six days
after vaccination |
Vial I9 (freezer A & B) given to Kyriaki Nomikou
(Pirbright) on 27th Jan 2010
*
Tubes I1and I10 were removed from the collection
*
|
|
2002 |
|
BOS2002/04 |
BTV Type 9
(Identified as
BTV by: ELISA.
Serotype identified by SNT) |
Village:
Milici Town: Vlasenica State/Province: Republike Srpska Country: Bosnia
Herzegovina
(13-14/8/02)
|
sheep
(IAH Pirbright
07/09/2002 Sue Graham) |
Shelf C2,
Box C2β,
Rows I1-J10
Added to collection on
25th Oct 2005 |
(Bos 890)
[ R13-890]
|
BHK
From blood
( E1/BHK1)
[IAH/BHK2] |
Dr Violeta
Santrac Veterinary Institute,"Dr Vaso Butozan", Banja Luka |
Grown in BHK
cells, then stored at -70° C
|
[S10: ] |
|
BOS2002/03 |
BTV Type 9
(Identified as
BTV by: ELISA.
Serotype identified by SNT )
|
Village:
Milici Town: Vlasenica State/Province: Republike Srpska Country: Bosnia
Herzegovina (13-14/8/02) |
Isolated from sheep
(Isolate made IAH Pirbright
7th Sept 2002 by Sue Graham) |
Shelf C4,
box C4γ
rows E1 - F10
(added to
collection
8th Dec 2005)
|
(877)
[ R13-877]
|
Adapted to BHK
cells
Isolated from blood
E1/BHK1
[IAH/BHK2] |
Dr Violeta
Santrac
Veterinary Institute, "Dr Vaso Butozan", Banja Luka |
Grown in BHK
cells, then stored at -70° C
|
[S10:
] |
|
BOS2002/02 |
BTVType 9
(Identified as
BTV by: ELISA.
Serotype identified by SNT and confirmed by Seg-2 RT-PCR and sequence
analysis) |
Village:
Milici Town: Vlasenica State/Province: Republike Srpska Country: Bosnia
Herzegovina
(13-14/8/02)
|
sheep
(IAH Pirbright
03/09/2002 by Sue Graham) |
Shelf B5,
Box B5β,
Rows A1-B10
Added to collection on 1st Aug 2005
|
876)
[ R13-876]
|
BHK
From blood
(E1/BHK1)
[IAH/BHK2] |
( Dr
Violeta Santrac Veterinary Institute,"Dr Vaso Butozan", Banja Luka |
*
Grown in BHK
cells, then stored at -70° C
* [
S6:AJ586687, S8: AM900377,
S9:
EU220294]
*
Vial positions
E1 (p)
were given to Eva
Veronesi (IAH Pirbright on 13th May 2010 for Ph.D studies).
one of these was retained in Misc
box C, bottom of freezer A
position E3
* |
|
BOS2002/01 |
BTV Type 9
(Identified as BTV by: ELISA
Serotype identified by SNT) |
Village: Milici
Town: Vlasenica State/Province: Republike Srpska Country: Bosnia
Herzegovina
(Sample
taken 13-14/8/02)
|
isolated from sheep
(isolate
made
9th Sept 2002
at IAH
Pirbright by Sue Graham)
|
Shelf C4,
box C4γ
rows C1 - D10
(added to
collection
8th Dec 2005)
|
(Bos 875)
[ R13-875]
|
Adapted to BHK
cells
Isolated from blood
E1/BHK1
[IAH/BHK2] |
Dr Violeta
Santrac Veterinary Institute,"Dr Vaso Butozan", Banja Luka |
[S5:
AM778443] |
|
2001 |
|
KOS2001/04 |
BTV-9
(Identified as BTV by: ELISA
Serotype identified
by SNT) |
Kosovo
2001 |
Sheep
(isolated by Sue
Graham at IAH Pirbright) |
Shelf A6,
BoxA6α,
Rows C1-D10
|
|
From blood
sample
[IAH/E1/BHK1] |
Philip Deng
&
Dr Artan Osmani
[R17/01] |
Tube C1
(freezer B) given to Eva Veronesi (Pirbright) 27th Sept 2005
*
Tube D1 (freezer A and B)
given to Kyriaki Nomikou
(Pirbright) 20th Jan 2010
*
Tube D2 was removed from freezer B
*
Tube D3 was removed from freezer A&B
* |
|
KOS2001/03 |
BTV Type 9
(Identified as BTV by: ELISA
Serotype identified
by SNT) |
municipality of Podujevo (30km north of Pristina), village
of Sllatina. Samples collected on 30/08/2001 |
Sheep
(isolated by Sue
Graham at IAH Pirbright) |
Shelf A6,
BoxA6α,
Rows I1-J10 |
No.34688 |
From blood
sample
[IAH/E1/BHK4] |
Philip Deng
&
Dr Artan Osmani
[R17/01] |
Vial I1 (freezer A and B)
given to Kyriaki Nomikou
(Pirbright)
20th Jan 2010 |
|
KOS2001/02 |
BTV Type 9
(Identified as BTV by: ELISA
Serotype identified
by SNT) |
Municipality of Glogovac (35km west of Pristina), village
of Sankovc, Kosovo Samples collected on 12/09/2001 |
Sheep
(isolated by Sue
Graham at IAH Pirbright) |
Shelf A5,
Box A5γ,
Rows C1-D10 |
No.34681 |
From blood
sample
[IAH/E1/BHK1]
|
Philip Deng
&
Dr Artan Osmani
[R17/01] |
|
|
KOS2001/01 |
BTV Type 9
(Identified as BTV by: ELISA
Serotype identified
by SNT) |
municipality of
Viti (35km southeast of Pristina), village of Sadovine.
Sample collected
on 12/09/2001 |
Sheep
(isolated by Sue
Graham at IAH Pirbright) |
Shelf A6,
BoxA6α,
Rows G1-H10 |
No.06244 |
From blood
sample
[IAH/E1/BHK2] |
Philip Deng
&
Dr Artan Osmani
[R17/01] |
*
Vial position
G1 from freezer A and B given to Kiki
Nomikou (Pirbright) for sequencing studies (7th Oct 2010).
*
|
|
SER2001/02 |
BTV Type 9
(Identified as BTV by: ELISA)
|
Serbia
(07/10/01) |
sheep |
Shelf A1,
Box A1γ,
Rows I1-J10 |
[R17/34678] |
E1/BHK2 |
|
|
[ ] |
|
SER2001/01 |
BTV Type 9
(Identified as BTV by: ELISA. Type confirmed by:
Seg 2 RT-PCR and sequence analyses )
|
Serbia
( 07/10/01 ) |
sheep
|
Shelf B5,
Box B5β,
Rows E1 - F10
added to collection on 1st Aug 2005 |
[R17/06547] |
E1/BHK2
[IAH/BHK2] |
|
[ S6:AJ586688] |
|
TUR2001/03 |
BTV-9
Identified by
BTV group-specific
Real time RT-PCR
[2]
Typed by
conventional RT-PCR [1]
and type specific Real-Time PCR
|
Turkey
Sample collected in 2001
|
From sheep blood
|
Shelf B14,
box B14
γ
,
rows I1-J10
Added to collection
25th Jan 2010
|
Iso15 |
From Blood adapted to Vero cells
ECE/BHK/Vero
[IAH/BHK1]
|
(c)
|
|
|
TUR2001/02 |
BTV-9
Identified by
BTV group-specific
Real time RT-PCR
[2]
Typed by
conventional RT-PCR [1]
and type specific Real-Time PCR
|
Turkey
Sample collected in 2001
|
From sheep blood
|
Shelf B14,
box B14
γ
,
rows G1-H10
Added to collection
25th Jan 2010
|
Iso14 |
From Blood adapted to Vero cells
ECE/BHK/Vero
[IAH/ BHK1]
|
(c)
|
|
|
TUR2001/01 |
BTV-9
Identified by
BTV group-specific
Real time RT-PCR
[2]
Typed by
conventional RT-PCR [1]
and type specific Real-Time PCR
|
Turkey
Sample collected in 2001
|
From sheep blood
|
Shelf B14,
box B14
γ
,
rows E1-F10
Added to collection
25th Jan 2010
|
Iso13 |
From Blood adapted to Vero cells
ECE/BHK/Vero
[IAH/BHK1]
|
(c)
|
|
|
2000 |
|
TUR2000/12 |
BTV Type 9
(Type confirmed
by:
Seg 2 RT-PCR) |
Turkey
(year 2000) |
|
Shelf A4,
Box A4α
Positions
G1-H10 |
BTTR 2000/12
[R13/00 no
12] |
[IAH-BHK2] |
Dr. Nahit
Yazicioglu,
Director , Ministry of agriculture and rural affairs,
Etlik
veterinary control and research institute
06020 ETLIK-Ankara/Turkey
6th Oct 2000 |
» contents
[S10: ] |
|
TUR2000/11 |
BTV Type 9
(Type confirmed
by:
Seg 2 RT-PCR) |
Turkey
(year 2000)
|
|
Shelf A4,
Box A4α
rows
E1-F10 |
BTTR 2000/11
[R13/00 no 11] |
[IAH-BHK2] |
Dr. Nahit
Yazicioglu,
Director , Ministry of agriculture and rural affairs,
Etlik
veterinary control and research institute
06020 ETLIK-Ankara/Turkey
6th Oct 2000 |
[S10:
] |
|
TUR2000/05 |
BTV Type 9
(Type confirmed
by:
Seg 2 RT-PCR
and sequence analyses )
|
Manisa,
Turkey
(year
2000) |
|
Shelf B1,
box, B1γ,
rows I1-J10
February 2005
|
BTTR 2000/5
[R13/00 no 5] |
(E3/BHK6)
[IAH/BHK1] |
Dr. Nahit
Yazicioglu, Director , Ministry of agriculture and rural affairs, Etlik
veterinary control and research institute 06020 ETLIK-Ankara/Turkey
6th Oct 2000 |
Sample Nos. 1/10g(a+b) were given to
Natalie Ross-Smith (IAH Pirbright) for sequencing studies,
7th March
2006 |
» contents
[S6:AJ586686] |
|
TUR2000/04 |
BTVType 9
(Type confirmed
by:
Seg 2 RT-PCR
and sequence analyses )
|
Denizli,
Turkey
(year
2000) |
|
Shelf C3,
box C3α,
rows I1 - J10
(added to
collection on
15th Nov 2006)
|
(BTTR 2000/4)
[R13/00 no 4] |
(E3/BHK7)
[IAH/BHK2] |
Dr. Nahit
Yazicioglu, Director , Ministry of agriculture and rural affairs, Etlik
veterinary control and research institute 06020 ETLIK-Ankara/Turkey
6th Oct 2000 |
|
» contents
[S6:AJ586685
] |
|
TUR2000/03 |
BTV Type 9
(Identified as
BTV by: ELISA. Type confirmed by:
Seg 2 RT-PCR
and sequence analyses )
|
Turkey
MANISA
(year 2000)
|
|
Shelf C3,
box C3β,
rows G1 -H10
(added to collection on 18th Nov
2005) |
BTTR 2000/3
[R13/00 No
3] |
E2/BHK7
[IAH/BHK2] |
Dr. Nahit
Yazicioglu, Director , Ministry of agriculture and rural affairs, Etlik
veterinary control and research institute 06020 ETLIK-Ankara/Turkey
6th Oct 2000 |
|
[S2:
AJ585175,
S5:AM778445,
S6:AJ586684
S8:
AM900376] |
|
GRE2000/02 |
BTV Type 9
(Identified as BTV by: ELISA. Type confirmed by:
Seg 2 RT-PCR
and sequence analyses )
|
Greece
(22/02/00) |
Sheep
|
Shelf B5,
Box B5β,
Rows G1 - H10
added to collection on 1st Aug 2005
|
(408/99) |
E2/BHK4
[IAH/BHK1] |
|
|
[S2:
S10:
] |
|
1999 |
|
TUR1999/06 |
BTV-9
Identified by BTV group-specific
Real time RT-PCR
[2]
Typed
by
conventional RT-PCR
[1]
and type specific Real-Time PCR
|
Turkey
Sample collected in 1999
|
From sheep blood
|
Shelf B15,
box B15
β
,
rows A1-B10
Added to collection
29th
Jan 2010
|
Iso11 |
From Blood adapted to Vero cells
ECE/BHK/Vero
[IAH/ ]
|
(c)
|
|
|
TUR1999/05 |
BTV-9
Identified by BTV group-specific
Real time RT-PCR
[2]
Typed by
conventional RT-PCR
[1]
and type specific Real-Time PCR
|
Turkey
Sample collected in 1999
|
From sheep blood
|
Shelf B15,
box B15
α
,
rows E1-F10
Added to collection
25th Jan 2010
|
Iso9 |
From Blood adapted to Vero cells
ECE/BHK/Vero
[IAH/ BHK1]
|
(c)
|
|
|
TUR1999/04 |
BTV-9
Identified by BTV group-specific
Real time RT-PCR
[2]
Typed
by
conventional RT-PCR
[1]
and type specific Real-Time PCR
|
Turkey
Sample collected in 1999
|
From sheep blood
|
Shelf B15,
box B15
α
,
rows I1-J10
Added to collection
29th
Jan 2010
|
Iso8 |
From Blood adapted to Vero cells
ECE/BHK/Vero
[IAH/ ]
|
(c)
|
|
|
TUR1999/03 |
BTV Type 9
Identified as BTV type 9
by
RT-PCR
|
Turkey
Sample collected in June 1999
|
From sheep blood
|
Shelf
D17
Box
D17ɑ
Rows D1-D9
(Added to the
collection on
21st
Nov 2014)
&
Shelf B15,
boxB15 Beta,
rows I1-J10
|
Iso7 |
From Blood adapted to Vero cells
ECE/BHK/Vero
[IAH/BHK1 ]
|
(c)
|
Only 9 vials stored in each freezer
|
|
TUR1999/02 |
BTV-9
Identified by BTV group-specific
real time RT-PCR
[2]
Typed by
conventional RT-PCR [1]
and type specific Real-Time PCR
|
Turkey
Sample collected in 1999
|
From sheep blood
|
Shelf B14,
box B14
γ
,
rows C1-D10
Added to collection
25th Jan 2010
|
Iso4 |
From Blood adapted to Vero cells
ECE/BHK/Vero
[IAH/ BHK1]
|
(c)
|
|
|
TUR1999/01
|
BTV Type 9
Identified as BTV type 9
by
RT-PCR
|
Turkey
Sample collected in
August 1999
|
from sheep blood
|
Shelf D17
Box D17ɑ
Rows C1-C10
(Added to
the
collection on
21st Nov 2014)
&
Shelf B15,
boxB15 Beta,
rows C1-D10
|
Iso1
|
From Blood adapted to Vero cells
ECE/BHK/Vero
[IAH/ BHK1]
|
(c)
|
|
|
GRE1999/32 |
BTV9 Identified by BTV group-specific Seg-7 RT-PCR and
Real time RT-PCR
[2].
Typed by
SNT,
convetional RT-PCR
[1]
and specific real-time PCR
|
GREECE:
Lesbos,
Aghia Paraskevi
(Central island)
39° 25' N
26°
27'
E
( sample collected on
25th Nov 1999)
|
isolated from
cattle spleen,
at IAH Pirbright
by: Nomikou K
|
Shelf C8,
Box C8α
Rows I1-J10
(added to
collection
25/08/2010
|
GR 732a/99LB
|
From cattle spleen
[IAH KC1/BSR1]
|
|
Derived from same animal as isolate GRE1999/30
and GRE1999/31
Animal in the same herd as isolate
GRE1999/29
|
|
GRE1999/31 |
BTV9
Identified by BTV group-specific Seg-7
RT-PCR and
Real time RT-PCR
[2]
Typed
by SNT,
convetional RT-PCR
[1]
and specific real-time PCR |
GREECE:
Lesbos,
Aghia Paraskevi
(Central island)
39° 25' N
26°
27' E
( sample collected on
25th Nov 1999) |
isolated from
cattle spleen,
at IAH Pirbright
by: Nomikou K.
|
Shelf B14,
box B14β
,
rows I1-J10
Added to collection
20th Jan 2010
|
GR 732a/99LB |
From cattle spleen
[IAH KC1]
|
|
Derived from same animal as isolate GRE1999/30
Used to generate
GRE1999/32
Animal in the same herd as isolate
GRE1999/29 |
|
GRE1999/30 |
BTV9
Identified by BTV group-specific Seg-7
RT-PCR and
Real time RT-PCR
[2]
Typed
by SNT,
convetional RT-PCR
[1]and specific real-time PCR |
GREECE:
Lesbos,
Aghia Paraskevi
(Central island)
39° 25' N
26°
27' E
( sample collected on
25th Nov 1999) |
isolated from
cattle
lymphnode
at IAH Pirbright
by: Nomikou K.
|
Shelf B14,
box B14β
,
rows G1-H10
Added to collection
20th Jan 2010
|
GR 732a/99LB |
From cattle
lymphnode
[IAH KC2]
|
|
Derived from same animal as isolate GRE1999/31
Animal in the same herd as isolate
GRE1999/29 |
|
|
GRE1999/12 |
BTV Type 9
Identified as BTV by ELISA / RT-PCR,
&
as type 9 by neutralisation assay |
Ierissos,
Chalkidiki,
Greece
(N. Central)
[40º 24΄Ν,
23º 52΄Ε]
29th Sept. 1999 |
Sheep (blood)
At : Centre of Athens Vet.
Institutions, Virus Lab, Athens.
By: K.Nomikou &
O. Mangana |
Shelf B1,
box B1γ,
rows A1-B10
Added to collection on 30th Sept
2004 |
GR408/99ChS |
BHK 21 CELLS
from blood
(E2BHK4)
[IAH/BHK2] |
Dr. Nomikou Kiki Centre of Athens Vet.
Institutions, Virus Lab, Athens. |
Seg-10 sequenced
at E2/BHK7 |
[Seg3:DQ186805
Seg10:AY449651] |
|
GRE1999/06 |
BTV Type 9 |
Chalkidiki,
Greece
[40º 24΄Ν,
23º 52΄Ε]
(1999) |
Sheep
|
Shelf A3,
box A3α,
rows I1-J10
(January 2003) |
(GR-408/99ChS) |
From blood
[IAH-BHK4]
|
Dr. Charalabos
Billinis Aristotle University Thessalonik |
Tubes
I1 and J1 (freezer A&B) given to Eva Veronesi
(Pirbright) on 27th Sept 2005
*
Tubes J2 (freezer A&B) removed from collection)
*
Tubes
J3 (freezer A&B) given
to T.Jabbar (Pirbright)
on 25th Jan 2008
* [S10:
] |
|
BUL1999/01 |
BTV Type 9
(Identified as BTV by: ELISA.
Type confirmed by:
Seg 2 RT-PCR
and sequence analyses )
|
Bulgaria
(29/09/99) |
NK |
Shelf A4,
Box A4γ,
Rows C1-D10 |
(TR 99-1; V8/99) |
(E1/BHK3)
[IAH-BHK2] |
|
|
[S3: DQ186797
S5:
AM778444] |
|
1998 |
|
GRE1998/01
|
BTV-9
Identified as BTV by ELISA and
RT-PCR,
as type 9 by serum neutralisation |
Afandou,
Rhodes,
Greece
(SE)
[36º 17΄Ν,
28º 08΄ Ε]
(3rd
Nov 1998)
|
sheep (blood
At :
Centre of Athens Vet. Institutions, Virus Lab, Athens. By:Dr. K.Nomikou
and O.Mangana |
Shelf B2,
Box B2α,
Rows A1-B10
added to collection on
30th Sept.2004 |
GR199/7a/98RS
(Animal code No. 7a) |
BHK 21 cells
BLOOD
(E2BHK9)
[IAH-BHK1] |
Nomikou Kiki Centre of Athens Vet.
Institutions, Virus Lab,
Athens. |
Seg-10 sequenced at E2/BHK11
[S3:DQ186808
S10:AY677629]
Vials position A1 given to K. Nomikou (IAH-P) for sequencing 22nd July
2009
Vial position A2 (g) from freezer A given to
Kyriaki Nomikou 24-APR-2012 to prepare RNA to be sent to Sanger Centre
for sequencing. The corresponding tube was not removed from
freezer B |
|
TUR1998/06 |
BTV-9
Identified by
BTV group-specific
Real time RT-PCR
[2]
Typed by
conventional RT-PCR [1]
and type specific Real-Time PCR
|
Turkey
Sample collected in 1998
|
from sheep blood
|
Shelf B15,
box B15
α
,
rows A1-B10
Added to collection
25th Jan 2010
|
Iso12 |
From Blood adapted to Vero cells
ECE/BHK/Vero
[IAH/ BHK1]
|
(c)
|
|
|
TUR1998/05 |
BTV-9
Identified by
BTV group-specific
Real time RT-PCR
[2]
Typed by
conventional RT-PCR [1]
and type specific Real-Time PCR
|
Turkey
Sample collected in 1998
|
from sheep blood
|
Shelf B15,
box B15
α
,
rows C1-D10
Added to collection
25th Jan 2010
|
Iso10 |
From Blood adapted to Vero cells
ECE/BHK/Vero
[IAH/BHK1]
|
(c)
|
|
|
TUR1998/04 |
BTV Type 9
Identified as BTV type 9
by
RT-PCR
|
Turkey
Sample collected in September 1998
|
from sheep blood
|
Shelf
D17
Box
D17ɑ
Rows B1-B10
(Added to the
collection on
21st
Nov 2014)
&
Shelf B15,
boxB15 Beta,
rows G1-H10
|
Iso6 |
From Blood adapted to Vero cells
ECE/BHK/Vero
[IAH/BHK1 ]
|
(c)
|
|
|
TUR1998/03 |
BTV Type 9
Identified as BTV type 9
by
RT-PCR
|
Turkey
Sample collected in 1998
|
from sheep blood
|
Shelf B15,
boxB15 Beta,
rows E1-F10 |
Iso5 |
From Blood adapted to Vero cells
ECE/BHK/Vero
[IAH/ ]
|
(c)
|
|
|
TUR1998/02
|
BTV-9
Identified by BTV group-specific
Real time RT-PCR
[2]
Typed by
conventional RT-PCR
[1]
and type specific Real-Time PCR
|
Turkey
Sample collected in 1998
|
from sheep blood
|
Shelf B15,
box B15
α
,
rows G1-H10
Added to collection
29th
Jan 2010
|
Iso3 |
From Blood adapted to Vero cells
ECE/BHK/Vero
[IAH/ ]
|
(c)
|
|
|
TUR1998/01
|
BTV-9
Identified by BTV group-specific
real time RT-PCR
[2]
Typed by
conventional RT-PCR [1]
and type specific Real-Time PCR
|
Turkey
Sample collected in 1998
|
from sheep blood
|
Shelf B14,
box B14
γ
,
rows A1-B10
Added to collection
25th Jan 2010
|
Iso2
|
From Blood adapted to Vero cells
ECE/BHK/Vero
[IAH/ BHK1]
|
(c)
|
|
|
1991 |
|
ISA1991/09 |
BTV-9 |
Indonesia
10/07/1991 |
|
Shelf D13, Box D13γ,
row J1 to J10
(Added to
collection
on 2nd
October 2014)
&
Shelf D16,
Box D16
α,
rows B1 to B10 |
KN (D15)
D15 |
[BHK3] |
|
|
|
1988 |
|
AUS1988/02
|
BTV-9
|
Australia
(1988)
|
|
|
Old isolate No.: Y920
Origin: DPP837
Sample sent as 'BTV-9'
|
??/2BHK21 (PP)
[IAH/??]
|
Peter Kirkland,
Elizabeth Macarthur Agricultural Institute,
Menangle, NSW 2568 Australia
Sent: 7th Sept 2010
|
Note
supplied with sample: CPE positive / BT-PCR positive
It is uncertain if the date given is sampling date or date of arrival at
EMAI.
ex BARC
(MTA) |
|
1985 |
|
AUS1985/01 |
BTV-9 |
Australia,
Northern
Territories
1985 |
(G. Gard) |
Shelf A6
Box A6β
Rows G1-H10 |
(DPP836,
8805-25-1105) |
E1/BHK3
[IAH-BHK1] |
Dr. Ian Pritchard
AAHL Geelong,
Australia |
*MTA* , not to be supplied to
3rd parties without written
consent from original source
[Seg3:DQ186790]
Aliquots H1 (freezer a & b)) given to Eva Veronesi (Pirbright)
27th Sept 2005
*
Vial H3 (freezer A&B) given
to Kyriaki Nomikou (Pirbright) 20th Jan 2010
*
Vial position H2 (freezer A) given to Kyriaki Nomikou 24-Apr-2012 to
prepare RNA for sequencing at the Sanger Centre
*
Vial
H4 was removed from freezer A
*
Vial
G1 was removed from freezer B.
|
|
1971 |
|
RSA1971/02 |
BTV-9
Identified by
serology |
Grown at IAH in
Dorset Poll sheep from a sample of virus originally isolated
between
1968-1972 |
|
|
(3080) |
no passage in
cell culture |
This virus has
been passed 4 times in sheep
It has been grown in Eggs and
the last two sheep passes were infected with freeze dried blood plus
infected egg material |
Freeze dried Virus in sheep
blood
(Dorset Poll) |
|
|
Unknown isolation
date |
|
TUR----/04 |
BTV 9
Identified
by seg-2 specific RT-PCR |
Turkey |
Isolated in
Turkey |
Shelf A17,
box A17 β,
rows G1–H10 |
|
[IAH BHK2] |
Supplied by Piet Van Rijn CIDC
Lelystad
[Stars
No. A140/08 no 63] |
Although the sample
came via Iran, it is believed that it originated from Turkey |
|
|
TUR----/01 |
BTV-9 |
Iran |
(isolated in
Iran) |
|
A140/08 number 63 |
KC cells
[IAH/BHK1] |
supplied by Dr. Mahmod Azimi
Razi Vaccine & Serum Research
Institute, Karaj, Iran P.O.BOX:31975/148 |
|
|
