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Isolates of African horse sickness virus (AHSV)

in the dsRNA virus collection at  IAH Pirbright 

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IAH dsRNA virus collection number: [Country code,  year / sample number]   rrrr = reference strain     vvvv = vaccine strain] Please send any requests for isolates from this collection to peter.mertens@bbsrc.ac.uk

Reference 
AHSV strains

 

Family: Reoviridaee,  Genus: Orbivirus,  Species:  African Horsesickness virus (AHSV)
Virus Species   *IAH dsRNA virus collection number:	Serotype   (identification method)	Place of original sample   eg country town [grid reference]     (date original sample was taken)	Isolated from which host species e.g Horse, Donkey, Zebra, etc   (Date of virus isolation, where, by whom )	Position of storage in reference freezers   (Date added to collection)	(Suppliers Isolate Designation)	Adapted to which cell types   Original sample material   (Passage history of sample)   [Passage history at TPI]	Original sample supplied to the collection by person / institution   [IAH virus diagnosis, ISIS /Stars sample number]	Comments CDNA clones, Purified virus (antigen)   in collection? References &  accession numbers
AHSV - untyped or multiple-types
SENvvv1/MV	Senegal   Types 1,2,3,4,5,6,7     Virus confirmed as  AHSV Types 1,2,3,4,5,6,7 by RT-PCR	AHSV multivalent(vaccine)		Shelf E3, box E3α, rows I1- J10    (added to collection 27th Sept 2007)	Polyequipeste vaccine Contre la peste equine Serotypes de 1a 7 Flacon de 10doses Voie sous cutanee A conserver a +4oC/ 20oC (usage veterinaire) Lot no. D-0107 Util.av Sep. 2009 ISRA vaccines Original bottled vaccine from supplier	[IAH/BHK1]	Yaya Thiongane, Laboratoire National de l’elevage Et de Recherches Veterinaires Bp 2057 Dakar Hann Senegal	(derived from SENvvvv/MV)   Virus confirmed as   AHSV Types 1,2,3,4,5,6,7 by RT-PCR
SENvvvv/MV	AHSV multivalent Types 1,2,3,4,5,6,7	(vaccine) Senegal)		1 vial only  Shelf E18 freezer A    (added to collection 27th Sept 2007)	Polyequipeste vaccine Contre la peste equine Serotypes de 1a 7 Flacon de 10doses Voie sous cutanee A conserver a +4oC/20oC (usage veterinaire) Lot no. D-0107 Util.av Sep. 2009 ISRA vaccines Original bottled vaccine from supplier Yaya Thiongane Laboratoire National de l’elevage Et de Recherches Veterinaires Bp 2057 Dakar Hann Senegal		Supplied by Yaya Thiongane Laboratoire National de l’elevage Et de Recherches Veterinaires Bp 2057 Dakar Hann Senegal	(used to generate SENvvv1/MV Virus confirmed as  AHSV Types 1,2,3,4,5,6,7 by RT-PCR
UAEvvvv/MV (UAEvvvv/01)	AHS-2, 6, 7 & 8	Polyvalent live  AHSV vaccine produced in Dubai		Shelf E5, box E5α, rows I1-J10,   (Added to collection on 16th Oct 2007)	Polyvalent live  AHSV vaccine - II	[IAH/BHK2]	Dr Ulli Wenery
UAEvvvv/01 (UAEvvv2/MV)	AHS 1,3 and 4	Polyvalent live AHSV vaccine produced in Dubai		Shelf E5, box  E5γ, rows A1-B10 (Added to collection  29th Oct 2007)	Polyvalent live  AHSV vaccine - I	[IAH/BHK2]	Dr Ulli Wenery
ETH2010/23	AHSV-2 and 9 Identified as AHSV-2 and 9 by Seg-2 specific  Real time RT-PCR  and sequencing	Equine Spleen Isolated in KC cells at IAH-P		Shelf  C11, box  C11β, rows G1-H9   Added to collection on 17th Dec 2010	09234/08	[IAH/KC1]	Melesse Balcha National animal health , diagnostic and investigation centre, Sebeta Ethiopia   Samples arrived 22nd Feb 2010   IAH Stars No. A18/10 02	Vial G10 removed  from freezer A *  Vials H10 removed  from freezer A & B Used to generate   ETH2010/21
ETH2010/21	AHSV-2 and 9 Identified as AHSV-2 and 9 by Seg-2 specific  real time RT-PCR  and sequencing	Equine Spleen  Isolated in KC cells at IAH-P		Shelf  C11, box  C11 γ, rows A1-B9 Added to collection on 17th Dec 2010	09234/08	[IAH/KC1/V1]	Melesse Balcha National animal health , diagnostic and investigation centre, Sebeta Ethiopia   Samples arrived 22nd Feb 2010   IAH Stars No. A18/10 02	Vial A10 were removed from freezer A & B  Derived from  ETH2010/23
ZIM2003/11	AHSV- untyped	Zimbabwe Sample collected on 26/03/2003			Horse (named Young Toddy) plasma BHK4 R4/03		Isolate collected by Stuart Gordon
RSA1960/01	AHSV-8 (plaque cloned)but also gives positive rRT-PCR for AHSV-5	Onderstepoort South Africa 10th May 1960			AHSV-8 (G)	MB3/BHK7	Supplied by  Rachel O'Hara, TPI [300692MB3]	Note:   Plaque cloned x3  (O'Hara et al 1998) strain plaque cloned three times at IAHduring 1991 in BHK cell monolayers
AHSV-1
RSArah1/01 (previously identified as RSArrah/01)	AHSV Type-1			Shelf B10,  box B10 β, row G1-H10   Added to collection on 22 Sept 2009	reference strain of AHSV-1 (A501)	(MB3) [IAH/BHK7]		Dr Baltus Erasmus OVI South Africa(1988) Howell (1962) Note: not Plaque cloned [ S1:AM883164, S2:AM883165, S3: AM883166, S4: AM883167, S5: AM883168,  S6: AM883169, S7: AM883170, S8: AM883171, S9: AM883172, S10: AM883173]
RSArah1/02	AHSV Type-1	27/01/91		Shelf C13, box C13 gamma,   tubes C1 to D10	Tube identified as AHSV-1 (c)MB3/BHK8 27/01/91	(MB3)[IAH/BHK10]		Note:  Plaque cloned  mycoplasma contaiminated
RSArah1/03	Confirmed as AHSV-1 by Seg-2 specific real-time RT-PCR  No contamination with other serotypes was detected)			Shelf C18, box C18γ  ows A1-B1		Current reference strain of AHSV-1	Supplied by  Rachel O'Hara, TPI MB3/BHK8 [IAH/BHK5]	Note: Plaque cloned three times at TPI in BHK  cell monolayers during 1991  (O'Hara et al 1998) * 2 x 1ml  A10(g) (from freezer A and B) were given to  Carrie Batten on 20th May 2013, for shipment to the Agri food Authority of Singapore
RSA1987/02	AHSV Type-1							(O'Hara et al 1998)
AHSV-2
RSArah2/01 (previously identified as RSArrah/02)	AHSV Type-2			Shelf B10,  box B10 β, row I1-J10   Added to collection on 22 Sept 2009	upplied as reference strain of AHSV-2 (OD)		Supplied by Dr Baltus Erasmus, OVI South Africa (1988)   (MB3) [IAH/BHK4]	Howell (1962)
RSArah2/02	AHSV Type-2			Shelf C13 , box C13γ  rows E1-F10				Note:  Plaque cloned  mycoplasma contaiminated
RSArah2/03	AHSV Type-2			Shelf C18,  box C18γ  rows C1-D10		Current reference strain of AHSV-2	Note:  Plaque cloned x3  (O'Hara et al 1998)	strain plaque cloned  three times at IAH in BHK cell monolayers during 1991 * 2 x 1ml  C10(g)  (from freezer A and B)   were given to Carrie Batten on 20th May 2013,  for shipment to the  Agri food and Veterinary Authority of Singapore
ETH2010/22	AHSV-2   Identified as AHSV-2 by Seg-2 specific  Real time RT-PCR  and sequencing	Ethiopia	From Equine Spleen Isolated in KC cells  at TPI	Shelf  C11, box  C11β, rows E1-F9   Added to collection  17th Dec 2010	29654/08	[IAH/KC1]	Melesse Balcha National animal health , diagnostic and investigation centre, Sebeta Ethiopia   Samples arrived 22nd Feb 2010   [TPI Stars No.A18/10 01]	I Vials E1 was removed  from freezer A * Vial F10 was removed from freezer A & B   Used to generate  ETH2010/20
ETH2010/20	AHSV-2 Identified as AHSV-2 by Seg-2 specific  Real time RT-PCR  and sequencing	Ethiopia	Equine  Spleen Isolated in KC cells  at TPI	Shelf  C11, box  C11β, rows  I1-J9   Added to collection  17th Dec 2010	29654/08	[IAH/KC1/V1]	Supplied by Melesse Balcha National animal health, diagnostic and investigation centre, Sebeta, Ethiopia. Samples arrived 22nd Feb 2010    [TPI Stars No. A18/10 01]	Vials J10 were removed  from freezer A & B Derived from ETH2010/22
ETH2010/02	AHSV-2  Identified as AHSV-2 by Seg-2 specific  Real time RT-PCR	Ethiopia 2010		NVI, ETHIOPIA Vaccine strain	Shelf  C7 Box  C7α Rows I1-J10   Added to collection  on  24 Aug 2010	ETH74/10  TPI Stars No.  A72/10 74	Supplied by  Esayas Gelaye, National Veterinary Institute 19 Debre Zeit Ethiopia. Samples arrived 22nd July 2010   Vero  [IAH/V1]	Vial J10 has been removed from freezer A
ETH2010/01	AHSV-2  Identified as AHSV-2 by Seg-2 specific  Real time RT-PCR	Ethiopia, Midie, Sample taken 21 June 2010		Equine Blood  Isolated in KC cells at IAH	Shelf  C7 box  C7α rows G1-H10   Added to collection on   24 Aug 2010	ETH11/10	[TPI / KC1]	Esayas Gelaye,  National Veterinary Institute 19 Debre Zeit Ethiopia Samples arrived 22nd July 2010 IAH Stars No. A72/10 11
ETH2010/01	AHSV-2  Identified as AHSV-2 by Seg-2 specific  Real time RT-PCR	Ethiopia, Midie, Sample taken 21 June 2010		Equine Blood  Isolated in KC cells at IAH	Shelf  C7 box  C7α rows G1-H10   Added to collection on   24 Aug 2010	ETH11/10	[TPI / KC1] [ IAH Stars No. A72/10 11]	Esayas Gelaye, National Veterinary Institute 19 Debre Zeit Ethiopia Samples arrived 22nd July 2010   Vials H1 to H3 removed from freezer A&B * Vials H4 removed  from freezer A
GHA2010/05	AHSV-2 identified  by Seg-2 RT-PCR and sequencing	Ghana Polo club		Equine spleen isolated at IAH Pirbright	Shelf B16 Box B16 α Rows G1-H10   (added to collection on 17th Mar 2010)	Suppliers designation 15	[IAH/KC1]	Andy Alhassan Veterinary services laboratory, Accra Ghana [A12/10 08] Clinical signs: respiratory symptoms   Outbreak reports
GHA2010/04	AHSV-2 identified  by Seg-2 RT-PCR and sequencing	Ghana Polo club		Equine spleen isolated at IAH Pirbright	Shelf B16 Box B16 α Rows E1-F10  (added to collection on 17th Mar 2010)	Suppliers designation 05	[IAH/KC1]	Andy Alhassan Veterinary services laboratory, Accra Ghana [A12/10 05] Clinical signs: respiratory symptoms Outbreak reports
GHA2010/03	AHSV-2 identified  by Seg-2 RT-PCR and sequencing	Ghana Polo club		Equine spleen isolated at IAH Pirbright	Shelf B16 Box B16 α Rows C1-D10 (added to collection on 17th Mar 2010)	Suppliers designation 02	[IAH/KC1]	Andy Alhassan Veterinary services laboratory, Accra Ghana [A12/10 02]  Clinical signs: respiratory symptoms Outbreak reports
GHA2010/02	AHSV-2 identified  by Seg-2 RT-PCR and sequencing	Ghana Polo club		Equine liver isolated at IAH Pirbright	Shelf B16 Box B16 α Rows A1-B10   (added to collection on 17th Mar 2010)		[IAH/KC1/BHK1]	Andy Alhassan Veterinary services laboratory, Accra Ghana [A7/10 06] Clinical signs: respiratory symptoms Same horse as GHA2010/01 Outbreak reports
GHA2010/01	AHSV-2 identified  by Seg-2 RT-PCR and sequencing	Ghana Polo club		Equine spleen isolated at IAH Pirbright	Shelf B15 Box B15 γ Rows I1-J10   (added to collection on 17th Mar 2010)		[IAH/KC1/BHK1]	Andy Alhassan Veterinary services laboratory, Accra Ghana [A7/10 05] Clinical signs: respiratory symptoms. Same horse as GHA2010/02 Outbreak reports
SEN2007/05	AHSV- 2 Identified as AHSV by serogroup specific RT-PCR   Typed by RT-PCR and sequence analysis of Seg-2	Diongo Village, Diourbel, Senegal   (Sample taken on 2nd Sept 2007)		Isolated from horse spleen: male six year old horse   (isolated at IAH Pirbright Sept 2007)	Shelf E5 Box E5 α Rows G1-H10     (added to collection on  16th Oct 2007)	No: 5	[IAH/MB1/BHK2]	A92/07 5    Dr Yaya Thiongane Chef du Laboratoire de Virologie,  Directeur de Recherches Chef du LNERV ISRA-LNERV BP 2057 Dakar-Hann Sénégal   * Duplicate sample from   freezer A & B provided to  Kasia Bankowska, IAH   11th July 2008 * Duplicate vials  positions G9 (G)  sent to Marta Vigo Martin, Algete Spain on  15th Dec 2009 under MTA * Outbreak data
SEN2007/04	AHSV- 2 Identified as AHSV by serogroup specific RT-PCR   Typed by RT-PCR and sequence analysis of Seg-2	Lerane Sambou Village, (Region of Fatick) Senegal   (Sample taken on 24thAug 2007)		Isolated from horse lung:   Female three year old horse   (isolated at IAH Pirbright Sept 2007)	Shelf E5, box E5 α, rows E1-F10   (added to collection on  16th Oct 2007)	No: 2	[IAH/MB1/ BHK3]	A92/07 2    Dr Yaya Thiongane Chef du Laboratoire  de Virologie, Directeur de Recherches, Chef du LNERV ISRA-LNERV, BP 2057, Dakar-Hann Sénégal
SEN2007/03	AHSV- 2 Identified as AHSV by serogroup specific RT-PCR   Typed by RT-PCR and sequence analysis of Seg-2	Diourbel, Senegal   (Sample taken on 26thAug 2007)		Isolated from horse spleen:  male six year old horse   (isolated at IAH Pirbright Sept 2007)	Shelf E5 Box E5 β Rows I1-J10     (added to collection on  16th Oct 2007)	No: 1	[TPI/MB1/BHK3]	A92/07 1   Dr Yaya Thiongane Chef du Laboratoire  de Virologie Directeur de Recherches Chef du LNERV ISRA-LNERV BP 2057 Dakar-Hann Sénégal
SEN2007/02	AHSV-2 Typed by RT-PCR and sequence analysis of Seg-2 Note: this isolate tested negative for BTV (Seg-1) and EHDV (Seg-9)  by real-time RT-PCR, and negative for FMDV by 3D assay and 5'UTR assay	Senegal2007		From horse spleen  (isolated at IAH Pirbright July 2007)   Note: From a horse that died showing typical clinical signs of AHSV.  From the same animal as SEN2007/01. The animal had been vaccinated 10 days earlier with AHSV-9	Shelf E1Box E1 βRows I1-J10(added to collection on  2nd July 2007)note (p)10/10b <1ml position I10 freezer B		Isolate provided by Yaya Thiongane Laboratoire National de l’elevage et de Recherches VeterinairesBp 2057 Dakar Hann Senegal-[A59/07 4] [IAH/MB1/BHK2]	Note:  (p)10/10b <1ml  in positions I10 & J10 , freezer B * Tube I9g (freezer A) was removed on  6th Sept 2010   *    Tube I9g (freezer A&B) was given to E. Veronesi (IAH-P) 26th April 2010 * Tube E1 A & B were given to Kasia Bankowska,  Pirbright  11th July 2008  *  Outbreak information
SEN2007/01	AHSV-2Typed by RT-PCR and sequence analysis of Seg-2	Senegal2007		From horse blood(isolated at IAH Pirbright July 2007)	Shelf E1 Box E1β Rows G1-H10 (added to collection on 2nd July 2007)		[IAH/MB1/BHK 2]	Yaya Thiongane Laboratoire National de l’elevage Et de Recherches VeterinairesBp 2057Dakar Hann Senegalnote (p)10/10b <1ml position H10 freezer B[ A59/07 1]From a horse that died showing typical clinical signs of AHSV From the same animal as SEN2007/02.  The animal had been vaccinated 10 days earlier with AHSV-9  Outbreak information
AHSV - 3
RSArrah/03	AHSV Type-3				Shelf B10,  box B10 γ, row A1-B10   Added to collection on 22 Sept 2009	reference strain of AHSV-3   (L)	(MB3) [IAH/BHK5]	Dr Baltus Erasmus,   OVI South Africa (1988) » Contents   Howell (1962)
RSArah3/02	AHSV Type-3				Shelf D7,  box D7 alpha, row E1-F10	reference strain of AHSV-3		Note:  Plaque cloned
RSArah3/03	AHSV Type-3   No contamination with other serotypes was detected by real-time RT-PCR)				Shelf C18, box C18γ  rows E1-F10	Current reference strain for AHSV-3		Note:  Plaque cloned x3  (O'Hara et al 1998) strain plaque cloned three times at IAH in BHK cell monolayers during 1991 * 2 x 1ml  E10(g) (from freezer A and B)  were given to Carrie Batten on 20th May 2013, for shipment to the Agri food and Veterinary Authority of Singapore
AHSV - 4
RSArrah/04	AHSV Type-4				Shelf B10,  box B10 γ, row C1-D10   Added to collection on 22 Sept 2009	reference strain of AHSV-4   (Vryheid)	(MB3) [IAH/BHK3]	Dr Baltus Erasmus,   OVI South Africa (1988) Howell (1962)
RSArah4/02	AHSV Type-4				Shelf D7,  box D7 alpha, row G1-H10	reference strain of AHSV-44	(MB3) [IAH/BHK7]	(Reference strain - plaque cloned )
SPArah4/03	AHSV Type-4   No contamination with other serotypes was detected by real-time RT-PCR)				Shelf C18, box C18γ  rows G1-H10	Current reference strain of AHSV-4	(MB3) [IAH/BHK7]	Note:  Plaque cloned x3  (O'Hara et al 1998) strain plaque cloned three times at IAH in BHK cell monolayers during 1991 from RSArrah/04 * 2 x 1ml  G10(g) (from freezer A and B)  were given to Carrie Batten on 20th May 2013, for shipment to the Agri food and Veterinary Authority of Singapore
ETH2010/08	AHSV-4  Identified as AHSV-4 by Seg-2 specific real time RT-PCR	Ethiopia		NVI, ETHIOPIA Vaccine strain	Shelf  C7, box  C7 γ, rows A1-B10 Added to collection  25 Aug 2010	ETH72/10	Vero [TPI/V1]	Esayas Gelaye, National Veterinary Institute 19 Debre Zeit Ethiopia Samples arrived 22nd July 2010 IAH Stars No. A72/10 72
ETH2010/07	AHSV-4  Identified as AHSV-4 by Seg-2 specific real time RT-PCR	Adama, Ethiopia   29 June 2010		Equine Blood, Isolated in KC cells at TPI	Shelf  C7, Box  C7β, rows I1-J10 Added to collection 25 Aug 2010	ETH50/10	[IAH/KC1]	Esayas Gelaye, National Veterinary Institute 19 Debre Zeit Ethiopia Samples arrived 22nd July 2010 IAH Stars No. A72/10 50Vial position I1 Freezer A&B have  been removed from the collection Vial position I10 Freezer A has been removed from the collection
ETH2010/06	AHSV-4  Identified as AHSV-4 by Seg-2 specific real time RT-PCR	Debre Zeit, Ethiopia Sample taken 24 June 2010		Equine Blood, Isolated in KC cells at TPI	Shelf  C7, Box  C7β, rows G1-H10 Added to collection  25 Aug 2010	ETH35/10	[IAH/KC1]	Esayas Gelaye, National Veterinary Institute 19 Debre Zeit Ethiopia Samples arrived 22nd July 2010 IAH Stars No. A72/10 35Vial position H10 Freezer A has been removed from the collection
ETH2010/05	AHSV-4  Identified as AHSV-4 by Seg-2 specific real time RT-PCR	Debre Zeit, Ethiopia  Sample taken 24 June 2010		Equine Blood,  Isolated in KC cells at IAH-P	Shelf  C7, box  C7β, rows E1-F10 Added to collection  25 Aug 2010	ETH34/10	[IAH/KC1]	Esayas Gelaye, National Veterinary Institute 19 Debre Zeit Ethiopia Samples arrived 22nd July 2010 IAH Stars No. A72/10 34Vial position F10 Freezer A has been removed from the collection
ETH2010/04	AHSV-4  Identified as AHSV-4 by Seg-2 specific real time RT-PCR	Ethiopia Gotia    Sample taken 24 June 2010		Equine Blood, Isolated in KC cells at IAH-P	Shelf  C7 Box  C7 β, Rows C1-D10 Added to collection  25 Aug 2010	ETH33/10	[IAH/KC1]	Esayas Gelaye, National Veterinary Institute 19 Debre Zeit Ethiopia Samples arrived 22nd July 2010 IAH Stars No. A72/10 33 * A single tube was removed from freezer B,  position C10 and given to Rhiannon silk (Pirbright)  for Culicoides transmission studies on 24th April 2013.  * Tubes D1 & D10  Freezer A have been removed from the collection
ETH2010/03	AHSV-4  Identified as AHSV-4 by Seg-2 specific real time RT-PCR	Ethiopia,   Wonji    Sample taken 21 June 2010		Equine Blood  Isolated in KC cells at TPI	Shelf  C7, box  C7β rows A1-B10   Added to collection  25 Aug 2010	ETH23/10	[IAH/KC1]	Esayas Gelaye, National Veterinary Institute 19 Debre Zeit Ethiopia   Samples arrived 22nd July 2010   IAH Stars No. A72/10 23Vial position B10 Freezer A has been removed
KEN2007/02	AHSV-4   Identified  by serotype specific RT-PCR against Seg 2 and confirmed by sequencing	Kenya   Sample taken October 2007		Equine Isolated from homogenised organs,  passed on Vero cell culture at CVRL Dubai   Isolated 22nd Jan 2008	Shelf A8 Box A8 γ Rows I1-J10    (added to collection on 4thApril 2008)	D1816/07	Vero2   [IAH/KC1]    Related to isolate KEN2007/01 but different passage history	Ulli Wernery CVRL Dubai     * Duplicate vial positions I10 (G) (from freezer A&B) sent to Marta Vigo Martin, Algete Spain on 15th Dec 2009 under MTA *
KEN2007/01	AHSV-4 Identified  by serotype specific RT-PCR	Kenya   Sample taken October 2007		Equine Isolated from homogenised organs,  passed on Vero cell culture at CVRL Dubai	Shelf A8,  Box A8 γ,  Rows E1-F10   Added to collection on  15th Jan 2008	D1816/07	Isolate provided by Ulli Wernery CVRL Dubai     V2 [IAH V2]	* Duplicate sample from freezer A & B provided to Kasia Bankowska, IAH  11th July 2008 * Vials F10 from freezer A & B provided to Kasia Bankowska, Pirbright 11th July 2008 * Vial E1 was removed from freezer A *  vials E10 were removed from freezer A&B.   Related to isolate KEN2007/02 but different passage history
SPA1988/01	AHSV-4	Spain 1988					BHK2
SPA1987/02	AHSV-4	Spain			Shelf B7, box B7α, rows E1- F10   (added to collection 12th Dec 2008)		BHK4 [IAH/BHK1]
SPA1987/01	AHSV-4	Spain  (Madrid)		Horse (named Ricarda)   (isolation date thought to be 21st Apr1989)	Shelf B7, box B7α, rows C1- D10   (added to collection 12th Dec 2008)		Blood   MB1 BHK 2 [IAH/BHK1]
RSA1938/01	AHSV-4 Confirmed as AHSV-4 single serotype by real-time Seg-2 specific RT-PCR	Vryheit Natal RSA 1938				Vryheit vaccine strain	original vaccine strain with up to 120 passages in mice [IAH/BHK5]	McIntosh 1958 Note:  Plaque cloned x3  (O'Hara et al 1998) strain plaque cloned three times at IAH in BHK cell monolayers during 1991
AHSV - 5
RSArrah/05	AHSV Type-5				Shelf B10,  box B10 γ, row E1-F10   Added to collection on 22 Sept 2009	reference strain of AHSV-5   (VH)	(MB3) [IAH/BHK6]	Dr Baltus Erasmus,   OVI South Africa (1988)   Howell (1962)
RSArah5/02	AHSV Type-5				Shelf C13 , box C13γ  rows G1-H10
RSArah5/03	AHSV Type-5   No contamination with other serotypes was detected by real-time RT-PCR)				Shelf C18, box C18γ  rows I1-J10	Current reference strain of AHSV-5		Note:  Plaque cloned x3  (O'Hara et al 1998) strain plaque cloned three times at IAH in BHK cell monolayers during 1991 From RSArrAH/05 * 2 x 1ml  I10(g) (from freezer A and B)  were given to Carrie Batten on 20th May 2013, for shipment to the Agri food and Veterinary Authority of Singapore *
AHSV - 6
RSArrah/06	AHSV Type-6				Shelf B10,  box B10 γ, row H1-I10   Added to collection on 22 Sept 2009	Supplied as reference strain of AHSV-6   (114)	(MB3) [IAH/BHK4]	Dr Baltus Erasmus,   OVI South Africa (1988)  Contents   Howell (1962)
RSArah6/02	AHSV Type-6				Shelf C14, BoxC14alpha, rows A1-B10
RSArah6/03	AHSV Type-6   No contamination with other serotypes was detected by real-time RT-PCR)				Shelf D7, box D7α.   rows I1-J10	Current reference strain of AHSV-6		Note:  Plaque cloned x3  (O'Hara et al 1998) strain plaque cloned three times at IAH in BHK cell monolayers during 1991 from RSArrah/06 * 2 x 1ml  I10(g) (from freezer A and B)  were given to Carrie Batten on 20th May 2013, for shipment to the Agri food and Veterinary Authority of Singapore
ETH2010/19	AHSV   Identified as AHSV by Seg-7 group-specific  real time RT-PCR     Typed  as AHSV-6 by sequencing of Seg-2	Ethiopia,   Yerer,     Sample taken 21 June 2010		From, equine blood  Isolated in KC cells at IAH-P	Shelf 9, box  C9α, rows C1-D10   Added  to collection on  31 Aug 2010	ETH08/10	[IAH/KC1]	Esayas Gelaye, National Veterinary Institute, 19 Debre Zeit, Ethiopia   Samples arrived 22nd July 2010   IAH StarsNo. A72/10 08Vial C1 removed from freezer A & B Vial C10 removed from freezer A
AHSV - 7
KENrrah/07	AHSV Type-7				Shelf B10,  box B10 γ, row I1-J10   Added to collection on 22 Sept 2009	reference strain of AHSV-7   (Karen)	(MB4) [IAH/BHK4]	Dr Baltus Erasmus,   OVI South Africa (1988) Howell (1962)
KENrah7/02	AHSV Type-7				Shelf D7,  box D7 alpha, row A1 - B10	reference strain of AHSV-7   (Karen)		Dr Baltus Erasmus,   OVI South Africa (1988) Howell (1962)
KENrah7/03	AHSV Type-7   No contamination with other serotypes was detected by real-time RT-PCR)				Shelf D7, box D7β   rows A1-B10	Current reference strain of AHSV-7		Note:  Plaque cloned x3  (O'Hara et al 1998) strain plaque cloned three times at IAH in BHK cell monolayers during 1991 * 2 x 1ml  A10(g) (from freezer A and B)  were given to Carrie Batten on 20th May 2013, for shipment to the Agri food and Veterinary Authority of Singapore
ETH2010/09	AHSV-7   Identified as AHSV-7 by Seg-2 type-specific  real time RT-PCR	Ethiopia		NVI, ETHIOPIA Vaccine strain	Shelf  C7, box  C7γ, rows C1-D10 Added to collection  26 Aug 2010	ETH73/10	Vero [IAH/V1]	Esayas Gelaye, National Veterinary Institute, 19 Debre Zeit, Ethiopia Samples arrived 22nd July 2010 IAH Stars No. A72/10 73-
SEN2007/06	AHSV 7   Typed by serotype specific RT-PCR	Guede Bousso Village Senegal   September 2007		Lung from male horse isolated in Vero cells in Senegal	Shelf A8, box A8γ, rows C1-D10   Added to collection on 15th Jan 2008	5	[V1/IAH V2]	Dr Malik Faye, Dakar, Senegal   [A117/07 5] * Tube D10 freezer A & B provided to Kasia Bankowska (Pirbright)  11th July 2008 * Tube D9 (freezer A&B) sent to Marta Vigo Martin, Algete Spain on 15th Dec 2009 under MTA * Tube C10 removed from freezer A&B
AHSV - 8
RSArrah/08	AHSV Type-8	Onderstepoort South Africa   (10th April 1960)		horse-2762   (virus isolated at OVI, South Africa)	Shelf B10,  box B11α, row A1-B10   Added to collection on 22 Sept 2009	reference strain of AHSV-8   (18/60)	Spleen   (MB3) [IAH/BHK2]	Dr Baltus Erasmus,   OVI South Africa (1988) Vial position G3, from freezer A and B, were supplied to the Estonia Veterinary and Food Laboratory, 28th July 2009    Howell (1962)
RSArah8/02	AHSV Type-8   No contamination with other serotypes was detected by real-time RT-PCR)				Shelf D7, box D7Alpha   rows C1-D10	reference strain of AHSV-8		Note:  Plaque cloned x3  (O'Hara et al 1998) strain plaque cloned three times at IAH in BHK cell monolayers during 1991 *
RSArah8/03	AHSV Type-8   No contamination with other serotypes was detected by real-time RT-PCR)				Shelf D7, box D7β   rows C1-D10	Current reference strain of AHSV-8		Note:  Plaque cloned x3  (O'Hara et al 1998) strain plaque cloned three times at IAH in BHK cell monolayers during 1991 derived from RSArrah/08 * 2 x 1ml  C10(g) (from freezer A and B)  were given to Carrie Batten on 20th May 2013, for shipment to the Agri food and Veterinary Authority of Singapore
ETH2010/11	AHSV-8    Identified as AHSV-8 by Seg-2 type-specific  real time RT-PCR	Ethiopia,   Debre Zeit    Sample taken 25 June 20100		Equine Blood Isolated in  KC cells at IAH	Shelf  C7 box  C7γ, rows G1-H10   Added to collection  26 Aug 2010	ETH37/10	[IAH/KC1]	Esayas Gelaye, National Veterinary Institute, 19 Debre Zeit, Ethiopia   Samples arrived 22nd July 2010   IAH Stars No. A72/10 37Vial G10 has been removed from Freezer A
ETH2010/10	AHSV-8  Identified as AHSV-8 by Seg-2 type-specific  real time RT-PCR	Ethiopia, Filtino   Sample taken 24 June 20100		Equine Blood, Isolated in  KC cells at TPI	Shelf  C7, box  C7γ, rows E1-F10 Added to collection  26 Aug 2010	ETH31/10	[IAH/KC1]	Esayas Gelaye, National Veterinary Institute, 19 Debre Zeit, Ethiopia Samples arrived 22nd July 2010 IAH Stars No. A72/10 31 Vials E1, E2, F1 have been removed from freezers A & B   *  Vial E3 has been removed from Freezer A
AHSV - 9
PAKrrah/09	AHSV Type-9	Pakistan Animal Husbandry Institute, Peshawar   (10th Dec 1959)		Horse [after inoculation with samples from donkeys]   (virus isolated at OVI, South Africa)	Shelf B10,  box B11α, row C1-D10   Added to collection on 22 Sept 2009	supplied as reference strain of AHSV-9   (7/60)	Spleen   (MB3) [IAH/BHK7]	Dr Baltus Erasmus,   OVI South Africa(1988)Spare sample position C1g freezer B*1 ml Sample from position C1g (A freezer only) sent to Pfizer/Fort Dodge (Joan de Plana) Spain 6th Sept 2010.  Corresponding vial in freezer B was not removed *Note: sample tested negative for BTV (Seg-1) and EHDV (Seg-9)  real-time RT-PCR, and negative for FMDV by 3D assay and 5'UTR assay* Howell (1962)
PAKrah9/02	AHSV Type-9				Shelf C13,  box C13γ, row I1-J10
PAKrah9/03	AHSV Type-8   No contamination with other serotypes was detected by real-time RT-PCR)	Pakistan Animal Husbandry Institute, Peshawar   (10th Dec 1959)			Shelf D7, box D7β   rows E1-F9	Current reference strain of AHSV-9		Note:  Plaque cloned x3  (O'Hara et al 1998) strain plaque cloned three times at IAH in BHK cell monolayers during 1991 * 2 x 1ml  E9(g) (from freezer A and B)  were given to Carrie Batten on 20th May 2013, for shipment to the Agri food and Veterinary Authority of Singapore * One vial position E10 g  was removed from freezer A on 27th February 2013 and given to Carrie Batten  (Pirbright)  for use by the ref lab .  The equivalent vial from freezer B was not removed *
SENvvvv/09	AHSV-9   (vaccine strain - typed as AHSV-9 by RT-PCR and sequence analyses of Seg-2)	Senegal			SShelf E1, Box E1 β, rows E1 to F10,   (added to collection 27th Sept 2007)	Monoequipeste vaccine Contre la peste equine Serotype 9 Flacon de 2 doses Voie sous cutanee A conserver a +4oc/20oc (usage veterinaire) Lot no. M-0107 Util.av Fev. 2009 ISRA vaccines	Original bottled vaccine from supplier	Yaya Thiongane Laboratoire National de l’elevage Et de Recherches Veterinaires Bp 2057 Dakar Hann Senegal   (used to generate SENvvv1/09) Vials E1, E10, F10 were removed from freezers A&B,  vial E9 was removed from freezer B, vial F9 was removed from freezer A.
SENvvv1/09	AHSV-9   (vaccine strain - type confirmed by RT-PCR and sequence analyses of Seg-2)	Senegal			Shelf E1 Box E1 β, rows E1-F10,   (added to collection on  27th June 2007)	Monoequipeste vaccine Contre la peste equine Serotype 9 Flacon de 2 doses Voie sous cutanee A conserver a +4oc/20oc (usage veterinaire) Lot no. M-0107 Util.av Fev. 2009 ISRA vaccines	Commercial vaccine [IAH/BHK1]	Yaya Thiongane Laboratoire National de l’elevage Et de Recherches Veterinaires Bp 2057 Dakar Hann Senegal   (derived from SENvvvv/09 ) Virus confirmed as  AHSV Type-9 by RT-PCR and sequence analysis of Seg-2
ETH2011/08	AHSV-9	Ethiopia			Shelf C18, box  C18α, rows G1 to H10
ETH2011/07	AHSV-9	Ethiopia			Shelf C18, box  C18α, rows E1 to F10
ETH2011/06	AHSV-9	Ethiopia			Shelf C18, box  C18α, rows C1 to D10
ETH2011/05	AHSV-9	Ethiopia			Shelf C18, box  C18α, rows A1 to B10
ETH2011/04	AHSV-9	Ethiopia	-		Shelf C17, box  C17 Gamma, rows I1 to J10
ETH2011/03	AHSV-9	Ethiopia			Shelf C17, box  C17 Gamma, rows G1 to H10
ETH2011/02	AHSV-9	Ethiopia			Shelf C17, box  C17 Gamma, rows E1 to F10
ETH2011/01	AHSV-9	Ethiopia			Shelf C17, box  C17 Gamma, rows C1 to D10
ETH2010/18	AHSV-9    Identified as AHSV-9 by Seg-2 type-specific real time RT-PCR	Ethiopia		NVI, Ethiopia    Vaccine strain	Shelf C9, box  C9α, rows A1-B10   Added to collection 31Aug 2010	ETH75/10	Vero   [IAH/V1]	Esayas Gelaye, National Veterinary Institute, 19 Debre Zeit, Ethiopia   Samples arrived 22nd July 2010   IAH Stars No. A72/10 75
ETH2010/17	AHSV-9    Identified as AHSV-9 by Seg-2 type-specific real time RT-PCR	Bonga, Ethiopia		Isolated  and Grown in Vero cells at NVI, Ethiopia	Shelf C8, box  C8γ, rows I1-J10   Added to collection 31Aug 2010	ET H56/10 (25/06/10)	Vero   [IAH/V1]	Esayas Gelaye, National Veterinary Institute, 19 Debre Zeit, Ethiopia   Samples arrived 22nd July 2010   IAH Stars No. A72/10 56Vial J10 from freezer A  was  removed from the collection
ETH2010/16	AHSV-9    Identified as AHSV-9 by Seg-2 type-specific real time RT-PCR	Ethiopia   Dembie,    Sample taken 3rd Jul 2010		Equine Lung Horse died of AHSV like symptoms  Isolated in KC cells at IAH	Shelf C8 Box C8γ, rows G1-H10   Added to collection 31Aug 2010	ET H70/10	[IAH/KC1]	Esayas Gelaye, National Veterinary Institute, 19 Debre Zeit, Ethiopia   Samples arrived 22nd July 2010 IAH Stars No. A72/10 70Note:  positions G10 freezer A&B was not filled when isolated was added to collection Vials H10 from freezer A and B were  removed from the collection Vial G9 was removed from freezer A Vial H9 was removed from freezer B
ETH2010/15	AHSV-9    Identified as AHSV-9 by Seg-2 type-specific real time RT-PCR	Ethiopia,   Debre Zeit,   Sample taken 15 June 2010		Equine Lung Horse died of AHSV like symptoms  Isolated in KC cells at IAH-	Shelf C8, box C8 γ, rows E1-F10   Added to collection 27Aug 2010	ET H68/10	[IAH/KC1]	Esayas Gelaye, National Veterinary Institute, 19 Debre Zeit, Ethiopia   Samples arrived 22nd July 2010 IAH Stars No. A72/10 68Note:  positions E10 freezer A&B was not filled when isolated was added to collection Vials E1, E2 & E3 from  freezer A& B were removed from the collection.
ETH2010/14	AHSV-9    Identified as AHSV-9 by Seg-2 type-specific real time RT-PCR	Ethiopia,   Godino   Sample taken 10 June 2010		Equine Lung Horse died of AHSV like symptoms  Isolated in KC cells at IAH-P	Shelf C8, box C8 γ, rows C1-D10   Added to collection 27Aug 2010	ET H66/10	[IAH/KC1]	Esayas Gelaye, National Veterinary Institute, 19 Debre Zeit, Ethiopia   Samples arrived 22nd July 2010 IAH Stars No. A72/10 66Note:  positions C10 freezer A&B was not filled when isolated was added to collection Vials C1, C2 & C3 from  freezer A& B were removed from the collection.
ETH2010/13	AHSV-9    Identified as AHSV-9 by Seg-2 type-specific real time RT-PCR	Ethiopia,   Akaki    Sample taken 21 June 2010		From equine Blood Isolated in KC cells at IAH	Shelf C8, box  C8 γ, rows A1-B10   Added to collection 27Aug 2010	ET H15/10	[IAH/KC1]	Esayas Gelaye, National Veterinary Institute, 19 Debre Zeit, Ethiopia   Samples arrived 22nd July 2010   IAH Stars No. A72/10 15Note: only 900ul per tube Sample B10 freezer A removed from collection
ETH2010/12	AHSV-9    Identified as AHSV-9 by Seg-2 type-specific real time RT-PCR	Ethiopia, Adama  Sample taken 29 June 2010		From equine Blood Isolated in KC cells at IAH	Shelf C7 Box  γ Rows I1-J10   Added to collection 27Aug 2010	ETH49/10	[IAH/KC1]	Esayas Gelaye, National Veterinary Institute, 19 Debre Zeit, Ethiopia   Samples arrived 22nd July 2010   IAH Stars No. A72/10 49Vial J10 has been removed from freezer A
GAM2009/04	AHSV 9 Identified by  Seg-2 specific RT-PCR and sequencing	Gambia,   Village Katana Omar -c-   Sample taken 22nd Oct 2009		Equine blood   Isolated at IAH Pirbright	Shelf B15 Box B15 γ Rows G1-H10  (added to collection on  1st Mar 2010)		[IAH/KC1/BHK1]	Philip Ivens Nixon Equine Vets Buckingham   [A89/09 18] From male horse age 3 unvaccinated No clinical signs Derived from GAM2009/02
GAM2009/03	AHSV 9 Identified by  Seg-2 specific RT-PCR and sequencing	Gambia,  Village Katana Omar -c-   Sample taken 22nd Oct 2009		Equine blood   Isolated at IAH Pirbright	Shelf B15 Box B15 γ Rows E1-F10   (added to collection on  1st Mar 2010)		[IAH/KC2/BHK1]	Philip Ivens Nixon Equine Vets Buckingham   [A89/09 12] From female horse age 3 unvaccinated No clinical signs Derived from  GAM2009/01
GAM2009/02	AHSV 9 Identified by  Seg-2 specific RT-PCR and sequencing	Gambia   Village Katana Omar -c-   Sample taken 22nd Oct 2009		Equine blood    Isolated at IAH Pirbright	Shelf B15 Box B15 γ Rows C1-D10   (added to collection on  1st Mar 2010)		[IAH/KC1]	Philip Ivens Nixon Equine Vets Buckingham   [A89/09 18] From male horse age 3 unvaccinated No clinical signs Used to generate GAM2009/04
GAM2009/01	AHSV 9 Identified by  Seg-2 specific RT-PCR and sequencing	Gambia   Village Katana Omar -c-   Sample taken 22nd Oct 2009		Equine blood   Isolated at IAH Pirbright	Shelf B15 Box B15 γ Rows A1-B10  (added to collection on  1st Mar 2010)		[IAH/KC2]	Philip Ivens Nixon equine vets Buckingham   [A89/09 12] From female horse age 3 unvaccinated No clinical signs Used to generate GAM2009/03
KEN2006/01	AHSV Type 9   Identified by RT-PCR and VNT	Kenya   The horse died on 25th July 2006		Isolated from a foal, which died in Nairobi. Typical lesions of AHS in the lung   Isolated in Dubai CVRL	Shelf D3, box D3 β, rows I1-J10   (added to collection  14th Nov 2006)	D1357/06	Ex trachea   Isolated in Vero cells   V1 [IAH/E1/BHK3]	Dr. Habil Ulrich Wernery Scientific Director Central Veterinary Research Laboratory P.O. Box 597, Dubai  U.A.E.   Received 25th Sept2006   (A86/06 1) From the same animal as KEN2006/02   Duplicate sample from freezer A & B provided to Kasia Bankowska, IAH  11th July 2008   » Contents
KEN2006/02	AHSV Type 9   Identified by RT-PCR and VNT	Kenya   The horse died on 25th July 2006		Isolated from a foal, which died in Nairobi. Typical lesions of AHS in the lung   Isolated in Dubai CVRL	Shelf D3, box D3 γ, rows A1-B10   (added to collection  14th Nov 2006)	D1357/06	Ex lung   Isolated in Vero cells   V1 [IAH/E1/BHK3]	Dr. Habil Ulrich Wernery Scientific Director Central Veterinary Research Laboratory P.O. Box 597, Dubai  U.A.E.   (A86/06 2)From the same animal as  KEN2006/01
SEN1998/01	AHSV-9 Identified as AHSV-9 by Seg-2 specific  Real time RT-PCR  and sequencing	Senegal		Equine  Isolated through Vero cells at ANSES	Shelf  C11, box  C11 α, rows I1-J10   Added to collection on  17th Dec 2010		V2 [IAH/BHK1]	Corinne Sailleau ANSES (AFFSA) France Vials I1 was removed from freezer A * Vial J10 was removed from freezer B

 

Miscellaneous AHSV samples
derived from  isolate number	Storage location	Notes
	Misc  box, Freezer A, Shelf E18, Box E18 β position G3	AHS ring-trial samples 12-07 - Freezer A, shelf E18, Miscellaneous box A, position G3.
	Misc  box, Freezer A, Shelf E18, Box E18 β position G2	AHS ring-trial samples 12-10 - Freezer A, shelf E18, Miscellaneous box A, position G2.
	Misc  box, Freezer A, Shelf E18, Box E18 β position G1	AHS ring-trial samples 12-09 - Freezer A, shelf E18, Miscellaneous box A, position G1.

H